Sphingosine-1-phosphate activates mouse vagal airway afferent C-fibres via S1PR3 receptors

The Journal of Physiology
Mayur J PatilBradley J Undem

Abstract

Sphingosine-1-phosphate (S1P) strongly activates mouse vagal C-fibres in the airways. Airway-specific nodose and jugular C-fibre neurons express mRNA coding for the S1P receptor S1PR3. S1P activation of nodose C-fibres is inhibited by a S1PR3 antagonist. S1P activation of nodose C-fibres does not occur in S1PR3 knockout mice. We evaluated the effect of sphingosine-1-phosphate (S1P), a lipid that is elevated during airway inflammatory conditions like asthma, for its ability to stimulate vagal afferent C-fibres in mouse lungs. Single cell RT-PCR on lung-specific vagal afferent neurons revealed that both TRPV1-expressing and TRPV1-non-expressing nodose neurons express mRNA coding for the S1P receptor S1PR3. TRPV1-expressing airway-specific jugular ganglion neurons also express S1PR3 mRNA. S1PR1 and S1PR2 mRNAs were also found to be expressed but only in a limited subset (32% and 22%, respectively) of airway-specific vagal sensory neurons; whereas S1PR4 and S1PR5 were rarely expressed. We used large scale two-photon imaging of the nodose ganglia from our ex vivo preparation isolated from Pirt-Cre;R26-GCaMP6s transgenic mice, which allows for simultaneous monitoring of calcium transients in ∼1000 neuronal cell bodies in the ganglia ...Continue Reading

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Citations

Sep 15, 2020·The Journal of Physiology·Mayur J PatilBradley J Undem
Mar 5, 2019·The Journal of Physiology·Thomas E Taylor-Clark
Oct 31, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hui SunBradley J Undem
Jan 8, 2021·Journal of Cellular Physiology·Xuehui FanGuozhong Li
Feb 16, 2021·Neuroscience Letters·Qihai Gu, Lu-Yuan Lee
Mar 6, 2021·Neuroscience Letters·Alexandra B PincusDavid B Jacoby
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mingwei YuShaoyong Yu

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