Sphingosine-1-phosphate inhibits actin nucleation and pseudopodium formation to control cell motility of mouse melanoma cells

FEBS Letters
S YamamuraY Igarashi

Abstract

Sphingosine-1-phosphate (Sph-1-P), the initial product of sphingosine (Sph) catabolism, has been reported to inhibit motility of mouse melanoma B16/F1 and other types of cells at very low concentrations (10-100 nM). Sph-1-P (100 nM-1 microM) inhibited pseudopodium formation by blocking polymerization and reorganization of actin filaments in newly formed pseudopodia, and reduced F-actin by approximately 25% in F1 cells. A pyrene-labeled actin nucleation assay revealed that Sph-1-P (100 nM) inhibits actin nucleation mediated by F1 cell plasma membranes. These results suggest that Sph-1-P interacts with molecules associated with actin nucleation to inhibit reorganization of pseudopodium formation and cell motility.

References

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Citations

Aug 31, 2001·Biochemical and Biophysical Research Communications·A HoriM Fujino
Dec 2, 1999·Experimental Cell Research·W H Moolenaar
Feb 9, 2010·Steroids·Natasha C Lucki, Marion B Sewer
Aug 8, 2006·Biochimica Et Biophysica Acta·Chiara Donati, Paola Bruni
Jun 23, 1997·FEBS Letters·D Meyer zu HeringdorfK H Jakobs
Jul 21, 1998·Annals of the New York Academy of Sciences·Y Igarashi

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