Sphingosine 1-phosphate inhibits activation of caspases that cleave poly(ADP-ribose) polymerase and lamins during Fas- and ceramide-mediated apoptosis in Jurkat T lymphocytes.

The Journal of Biological Chemistry
O CuvillierS Spiegel

Abstract

Ceramide, a sphingolipid generated by the hydrolysis of membrane-associated sphingomyelin, appears to play a role as a gauge of apoptosis. A further metabolite of ceramide, sphingosine 1-phosphate (SPP), prevents ceramide-mediated apoptosis, and it has been suggested that the balance between intracellular ceramide and SPP levels may determine the cell fate (Cuvillier, O., Pirianov, G, Kleuser, B., Vanek, P. G., Coso, O. A., Gutkind, J. S., and Spiegel, S. (1996) Nature 381, 800-803). Here, we investigated the role of SPP and the protein kinase C activator, phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), in the caspase cascade leading to the proteolysis of poly(ADP-ribose) polymerase (PARP) and lamins. In Jurkat T cells, Fas ligation or addition of exogenous C2-ceramide induced activations of caspase-3/CPP32 and caspase-7/Mch3 followed by PARP cleavage, effects that can be blocked either by SPP or TPA. Furthermore, both SPP and TPA inhibit the activation of caspase-6/Mch2 and subsequent lamin B cleavage. Ceramide, in contrast to Fas ligation, did not induce activation of caspase-8/FLICE and neither SPP nor TPA were able to prevent this activation. Thus, SPP, likely generated via protein kinase C-mediated activation of ...Continue Reading

References

Oct 1, 1994·The Journal of Experimental Medicine·M G CifoneR Testi
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y A LazebnikW C Earnshaw
Aug 1, 1994·The Journal of Experimental Medicine·A Haimovitz-FriedmanR N Kolesnick
Mar 19, 1993·Science·L M ObeidY A Hannun
Jan 26, 1996·The Journal of Biological Chemistry·J SchlegelD W Nicholson
Mar 1, 1996·The Journal of Biological Chemistry·A M ChinnaiyanV M Dixit
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·D L Vaux, A Strasser
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·J ZhangY A Hannun
Apr 1, 1996·Current Opinion in Cell Biology·S SpiegelR Kolesnick
Oct 21, 1996·FEBS Letters·N MizushimaN Miyasaka
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S M SrinivasulaE S Alnemri
Mar 21, 1997·The Journal of Biological Chemistry·Q ZhouG S Salvesen
Aug 1, 1997·Trends in Biochemical Sciences·D W Nicholson, N A Thornberry
Sep 26, 1997·The Journal of Biological Chemistry·A D TepperW J van Blitterswijk

❮ Previous
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Citations

Feb 19, 2000·Biochemical and Biophysical Research Communications·Y YamazakiH Ohta
Oct 18, 2000·Biochemical and Biophysical Research Communications·I K KimY K Jung
Oct 17, 2003·Archives of Pharmacal Research·Dong-Seok KimKyoung-Chan Park
Oct 30, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Marie-Laure RantyJean-Claude Quintyn
Oct 18, 2011·Cancer Metastasis Reviews·Jessie W YesterTomasz Kordula
Dec 31, 2003·Bioorganic & Medicinal Chemistry·Hayato NiiroKenji Ogino
Mar 17, 2004·Experimental Cell Research·Nicholas J Donato, Jim Klostergaard
Dec 5, 1998·Biochimica Et Biophysica Acta·D K Perry, Y A Hannun
Apr 28, 2001·Prostaglandins & Other Lipid Mediators·E J Goetzl
Apr 28, 2001·Prostaglandins & Other Lipid Mediators·A Olivera, S Spiegel
Nov 30, 2000·Immunology Today·E E Prieschl, T Baumruker
Jul 23, 2003·Cellular Signalling·Dong-Seok KimKyoung-Chan Park
Feb 19, 2000·Cellular Signalling·R T Dobrowsky
Oct 31, 1998·Current Opinion in Immunology·M E Peter, P H Krammer
Feb 1, 2000·Trends in Cell Biology·Y A Hannun, C Luberto
Jun 6, 2003·Bioorganic & Medicinal Chemistry·Keiji ShikataTaro Tachibana
Apr 5, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Timothy Hla
Apr 5, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Benedetta CinqueM Grazia Cifone
Apr 5, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Olivier Cuvillier, Thierry Levade
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·I SchmitzP H Krammer
Apr 13, 2000·Biochimica Et Biophysica Acta·S Spiegel, S Milstien
Jun 19, 2002·Biochimica Et Biophysica Acta·Hervé Le StunffSarah Spiegel
Jun 19, 2002·Biochimica Et Biophysica Acta·Wolfgang Siess
Jan 18, 2003·Biochimica Et Biophysica Acta·Jonathan L Tilly, Richard N Kolesnick
Jan 18, 2003·Biochimica Et Biophysica Acta·Olivier Cuvillier
Jan 18, 2003·Biochimica Et Biophysica Acta·Michael MaceykaSarah Spiegel
Apr 27, 1999·Biochimica Et Biophysica Acta·T Levade, J P Jaffrézou
Jul 6, 2000·The Biochemical Journal·S Pyne, N J Pyne
Mar 10, 2001·Journal of Neurochemistry·L C EdsallS Milstien
Aug 20, 2004·Biology of Reproduction·Z Roth, P J Hansen
Mar 9, 1999·Alcoholism, Clinical and Experimental Research·A SlomianyB L Slomiany

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