Abstract
This study shows that sphingosine 1-phosphate (S1P) exerts an anti-migratory action in C2C12 myoblasts by reducing directional cell motility and fully abrogating the chemotactic response to insulin-like growth factor-1. The anti-migratory response to S1P required ligation to S1P(2), being attenuated in myoblasts where the receptor was down-regulated by specific antisense oligodeoxyribonucleotides or small interfering RNA (siRNA) and conversely potentiated in S1P(2)-overexpressing myoblasts. The investigation of RhoA and Rac GTPases, critically implicated in cell motility regulation, demonstrated that RhoA was rapidly activated by S1P, while Rac1 was unaffected within the first 5 min but stimulated thereafter. RhoA, but not Rac activation, was identified as a S1P(2)-dependent pathway in experiments in which receptor expression was attenuated by siRNA treatment or up-regulated by S1P(2)-encoding plasmid transfection. Finally, by expression of the dominant negative mutant of RhoA, the GTPase was found implicated in the anti-migratory action of S1P, whereas modulation of Rac1 functionality unaffected the anti-chemotactic effect of S1P, ruling out a role for this protein in the biological response. Since S1P was previously shown to ...Continue Reading
References
Jul 1, 1995·The Journal of Cell Biology·K E BornfeldtS Hakomori
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Sep 2, 1997·Biochemistry·S YamamuraY Igarashi
Nov 11, 1999·Cell·M J LeeT Hla
May 5, 2000·The Biochemical Journal·T KimuraF Okajima
Feb 13, 2001·Journal of Cellular Physiology·A BarberoD M Noonan
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 28, 2002·Circulation Research·Yasuji RyuYoh Takuwa
Feb 18, 2003·Molecular and Cellular Biology·Naotoshi SugimotoYoh Takuwa
Jun 7, 2003·Progress in Lipid Research·Kenneth WattersonSarah Spiegel
Jun 14, 2003·The Biochemical Journal·Hironori YamaguchiYoh Takuwa
Jun 18, 2003·The Journal of Biological Chemistry·Kayo ArikawaYoh Takuwa
Sep 23, 2003·Biochimica Et Biophysica Acta·Elisabetta MeacciPaola Bruni
Oct 30, 2003·Journal of Cellular Physiology·L FormigliS Zecchi Orlandini
Mar 17, 2004·The Journal of Biological Chemistry·Brigitte Anliker, Jerold Chun
Jul 24, 2004·Seminars in Cell & Developmental Biology·Timothy Hla
Apr 27, 2005·Biochemical and Biophysical Research Communications·Tadayoshi KawataFumikazu Okajima
May 4, 2005·Cancer Research·Denise LepleyFernando Ferrer
Citations
Jan 30, 2008·The Journal of Biological Chemistry·Gyu-Un BaeRobert S Krauss
Jul 23, 2008·Molecular Cancer Therapeutics·Dongmei WangYan Xu
Jul 14, 2012·Skeletal Muscle·Caterina BernacchioniPaola Bruni
Jul 14, 2010·Stem Cell Reviews and Reports·Nao R KobayashiAlice Pébay
Aug 8, 2009·Cellular and Molecular Life Sciences : CMLS·Elena RapizziPaola Chiarugi
Aug 11, 2012·Biochimica Et Biophysica Acta·Chiara DonatiPaola Bruni
Mar 12, 2008·Biochemical and Biophysical Research Communications·Wen-Lei ZhuoZheng-Tang Chen
Apr 28, 2007·Stem Cells·Chiara DonatiPaola Bruni
Jun 11, 2011·Journal of Cellular Physiology·Xihong LiuLiying Li
Aug 19, 2014·The FEBS Journal·Francesca CencettiChiara Donati
Aug 8, 2006·Biochimica Et Biophysica Acta·Chiara Donati, Paola Bruni
May 9, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Anna LaurenzanaChiara Donati
Nov 28, 2012·The International Journal of Biochemistry & Cell Biology·Jelena KocićDiana Bugarski
Dec 20, 2011·Biochimica Et Biophysica Acta·Serena CalisePaola Bruni
Sep 29, 2011·Biochimie·Patricia GangoitiPaola Bruni
Dec 15, 2010·Journal of Hepatology·Changyong LiLiying Li
Jan 28, 2017·Nutrition & Metabolism·James F MarkworthDavid Cameron-Smith
Aug 15, 2017·PloS One·Sarah E NicholasDimitrios Karamichos
Jul 23, 2009·American Journal of Physiology. Endocrinology and Metabolism·Taku NedachiMakoto Kanzaki
Oct 26, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anabel S de la Garza-RodeaJulie D Saba
May 10, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brenda A BondesenGrace K Pavlath
Nov 19, 2019·Frontiers in Cell and Developmental Biology·John H NguyenJames G Ryall
May 6, 2008·Molecular and Cellular Biochemistry·Elena RapizziPaola Bruni
Aug 28, 2020·Frontiers in Endocrinology·Sophie Tan-ChenEric Hajduch
Aug 11, 2018·Cancer Research·Kristianne M OristianCorinne M Linardic
Feb 3, 2021·International Journal of Molecular Sciences·Caterina BernacchioniChiara Donati
Jul 16, 2021·Reproductive Medicine and Biology·Kirti R ChaharPankaj Goyal