Sphingosine 1-phosphate inhibits cell migration in C2C12 myoblasts

Biochimica Et Biophysica Acta
Laura BeccioliniPaola Bruni

Abstract

This study shows that sphingosine 1-phosphate (S1P) exerts an anti-migratory action in C2C12 myoblasts by reducing directional cell motility and fully abrogating the chemotactic response to insulin-like growth factor-1. The anti-migratory response to S1P required ligation to S1P(2), being attenuated in myoblasts where the receptor was down-regulated by specific antisense oligodeoxyribonucleotides or small interfering RNA (siRNA) and conversely potentiated in S1P(2)-overexpressing myoblasts. The investigation of RhoA and Rac GTPases, critically implicated in cell motility regulation, demonstrated that RhoA was rapidly activated by S1P, while Rac1 was unaffected within the first 5 min but stimulated thereafter. RhoA, but not Rac activation, was identified as a S1P(2)-dependent pathway in experiments in which receptor expression was attenuated by siRNA treatment or up-regulated by S1P(2)-encoding plasmid transfection. Finally, by expression of the dominant negative mutant of RhoA, the GTPase was found implicated in the anti-migratory action of S1P, whereas modulation of Rac1 functionality unaffected the anti-chemotactic effect of S1P, ruling out a role for this protein in the biological response. Since S1P was previously shown to ...Continue Reading

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Citations

Jan 30, 2008·The Journal of Biological Chemistry·Gyu-Un BaeRobert S Krauss
Jul 14, 2010·Stem Cell Reviews and Reports·Nao R KobayashiAlice Pébay
Aug 8, 2009·Cellular and Molecular Life Sciences : CMLS·Elena RapizziPaola Chiarugi
Aug 8, 2006·Biochimica Et Biophysica Acta·Chiara Donati, Paola Bruni
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Nov 19, 2019·Frontiers in Cell and Developmental Biology·John H NguyenJames G Ryall
May 6, 2008·Molecular and Cellular Biochemistry·Elena RapizziPaola Bruni
Aug 28, 2020·Frontiers in Endocrinology·Sophie Tan-ChenEric Hajduch
Feb 3, 2021·International Journal of Molecular Sciences·Caterina BernacchioniChiara Donati

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