Sphingosine-1-Phosphate Is a Crucial Signal for Migration of Retina Müller Glial Cells

Investigative Ophthalmology & Visual Science
María V SimónNora P Rotstein

Abstract

Migration of Müller glial cells is enhanced in proliferative retinopathies, but the mechanisms involved are ill defined. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid synthesized by sphingosine kinase (SphK), which promotes proliferation, migration, and inflammation, acting as an intracellular mediator and activating a family of membrane receptors (S1PRs). We investigated whether S1P regulated glial migration. Müller glial cell cultures from rat retinas were supplemented with 5 μM S1P, and migration was evaluated by scratch-wound assays. Cultures were treated with SphK inhibitor 2 (SphKI 2), a SphK1 inhibitor, or with W146 and BML-241, S1P1 and S1P3 antagonists, respectively, to investigate whether Müller glial cells synthesized S1P and S1P-activated S1PRs to stimulate migration. The effects of LY294002, U0126, and SB203580, which are phosphatidylinositol-3 kinase (PI3K), extracellular signal regulated kinase/mitogen-activated protein kinase (ERK/MAPK), and p38 MAPK inhibitors, respectively, on glial migration were determined. Sphingosine-1-phosphate addition prompted the formation of lamellipodia and enhanced glial migration. SphKI 2 almost completely prevented glial migration in controls; BML-241 inhibited this mi...Continue Reading

Citations

May 24, 2018·The European Journal of Neuroscience·Paula V SubiradaMaría C Sánchez
Oct 19, 2019·Science·Andreane Cartier, Timothy Hla
Jun 28, 2019·Frontiers in Cellular Neuroscience·M Victoria SimónNora P Rotstein
Oct 20, 2017·Journal of Neurochemistry·María A Zorrilla-ZubileteDafne M Silberman
Sep 20, 2020·Journal of Lipid Research·M Victoria SimonNawajes Mandal
Jan 8, 2021·Journal of Cellular Physiology·Xuehui FanGuozhong Li
Feb 9, 2021·Journal of Lipid Research·Simon M VictoriaMandal Nawajes
Nov 17, 2020·Experimental Eye Research·Marcela S VeraNora P Rotstein
May 9, 2021·The British Journal of Ophthalmology·Rasha A AlshaikhChristian Waeber
May 23, 2021·Experimental Eye Research·Natsuko NakamuraMakoto Aihara
Jul 17, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Yanel A VolontéOlga L German
Nov 30, 2021·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Rafael André da SilvaDânia Emi Hamassaki

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