Sphingosine-1-phosphate rapidly increases cortisol biosynthesis and the expression of genes involved in cholesterol uptake and transport in H295R adrenocortical cells

Molecular and Cellular Endocrinology
Natasha LuckiMarion B Sewer

Abstract

In the acute phase of adrenocortical steroidogenesis, adrenocorticotrophin (ACTH) activates a cAMP/PKA-signaling pathway that promotes the transport of free cholesterol to the inner mitochondrial membrane. We have previously shown that ACTH rapidly stimulates the metabolism of sphingolipids and the secretion of sphingosine-1-phosphate (S1P) in H295R cells. In this study, we examined the effect of S1P on genes involved in the acute phase of steroidogenesis. We show that S1P increases the expression of steroidogenic acute regulatory protein (StAR), 18-kDa translocator protein (TSPO), low-density lipoprotein receptor (LDLR), and scavenger receptor class B type I (SR-BI). S1P-induced StAR mRNA expression requires Gα(i) signaling, phospholipase C (PLC), Ca(2+)/calmodulin-dependent kinase II (CamKII), and ERK1/2 activation. S1P also increases intracellular Ca(2+), the phosphorylation of hormone sensitive lipase (HSL) at Ser(563), and cortisol secretion. Collectively, these findings identify multiple roles for S1P in the regulation of glucocorticoid biosynthesis.

References

Sep 25, 1992·Proceedings of the National Academy of Sciences of the United States of America·J J EganC Londos
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E DegermanV C Manganiello
Jul 20, 1990·European Journal of Biochemistry·A J Garton, S J Yeaman
Apr 1, 1994·Molecular and Cellular Endocrinology·William E RaineyJ I Mason
Jul 9, 1993·Science·R TaussigA G Gilman
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·K M CaronK L Parker
Jan 24, 1998·The Journal of Biological Chemistry·F ArakaneJ F Strauss
Feb 15, 2000·The Journal of Biological Chemistry·G M CliffordS J Yeaman
Apr 13, 2000·Biochimica Et Biophysica Acta·Sarah Spiegel, Sheldon Milstien
Jun 1, 2000·Biochimica Et Biophysica Acta·A HuwilerK Sandhoff
Apr 3, 2001·The Journal of Steroid Biochemistry and Molecular Biology·S B MeroniS B Cigorraga
Apr 28, 2001·Prostaglandins & Other Lipid Mediators·A Olivera, Sarah Spiegel
Sep 6, 2001·Naunyn-Schmiedeberg's Archives of Pharmacology·A BischoffM C Michel
Oct 3, 2001·The Journal of Biological Chemistry·A S GreenbergFredric B Kraemer
Dec 1, 2001·Science·Timothy HlaM J Kluk
May 16, 2002·The Journal of Biological Chemistry·Sarah Spiegel, Sheldon Milstien
May 16, 2002·The Journal of Biological Chemistry·Alfred H Merrill
Oct 5, 2002·Journal of Lipid Research·Fredric B Kraemer, Wen-Jun Shen
Feb 1, 2003·FEBS Letters·Miriam RábanoAntonio Gómez-Muñoz
May 3, 2003·Nature Reviews. Molecular Cell Biology·Sarah Spiegel, Sheldon Milstien
May 28, 2003·Microscopy Research and Technique·Marion B Sewer, Michael R Waterman
Sep 5, 2003·The Journal of Biological Chemistry·Maddalena IllarioMario Vitale
May 20, 2004·The Journal of Biological Chemistry·John M SchmittThomas R Soderling
May 26, 2004·Biochimica Et Biophysica Acta·Tarek A TahaLina M Obeid
Jul 20, 2004·Journal of Cellular Biochemistry·Teresa Sanchez, Timothy Hla
Nov 16, 2004·Molecular and Cellular Endocrinology·William E RaineyBernard P Schimmer
Apr 27, 2005·Endocrinology and Metabolism Clinics of North America·Wiebke Arlt, Paul M Stewart
Sep 27, 2005·Cellular Signalling·González-Yanes Carmen, Sánchez-Margalet Víctor
Mar 24, 2006·Journal of Lipid Research·Leyre BrizuelaAntonio Gómez-Muñoz
Sep 15, 2006·FEBS Letters·Besim Ogretmen
Oct 20, 2006·The Journal of Biological Chemistry·Jun LiuVassilios Papadopoulos
Oct 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Poulami MitraSarah Spiegel
Jan 9, 2007·Molecular and Cellular Endocrinology·Fredric B Kraemer
Jan 11, 2007·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S SchinnerW A Scherbaum
Jun 8, 2007·European Journal of Pharmacology·Yoshihiro AnadaAkio Kihara
Jun 26, 2007·Trends in Molecular Medicine·Youssef H Zeidan, Yusuf A Hannun
Sep 6, 2007·Drug Metabolism Reviews·Marion B SewerSrinath Jagarlapudi
Oct 2, 2007·Trends in Endocrinology and Metabolism : TEM·Sergio E AlvarezSarah Spiegel
Aug 30, 2008·Lipids·Marion B Sewer, Donghui Li
Oct 9, 2008·Molecular and Cellular Endocrinology·Margery A Connelly
Nov 15, 2008·Molecular and Cellular Endocrinology·Cecilia PoderosoErnesto J Podesta
Jan 20, 2009·Molecular and Cellular Endocrinology·Pulak R MannaDouglas M Stocco
Mar 27, 2009·Molecular Human Reproduction·Pulak R MannaDouglas M Stocco
Jan 30, 2010·The Journal of Biological Chemistry·Kazuaki TakabeSarah Spiegel
Mar 20, 2010·The Journal of Biological Chemistry·Laila SuhaimanSilvia A Belmonte
May 22, 2010·Journal of Biomedicine & Biotechnology·Jen-Hsiang TsaiChong-Kuei Lii

Citations

Apr 19, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Takayuki HaraHirofumi Makino
Nov 29, 2012·Molecular and Cellular Endocrinology·Marion B Sewer, Donghui Li
Feb 18, 2016·The Journal of Endocrinology·Wendy B Bollag
May 15, 2018·Endocrinology·Jinjiang FanVassilios Papadopoulos
Oct 4, 2017·The Journal of Endocrinology·Stephan WerthWalter Raasch
Oct 28, 2017·The Biochemical Journal·David R OwenVassilios Papadopoulos
Feb 7, 2017·The Journal of Clinical Investigation·Rathi PrasadLouise A Metherell
Jul 2, 2017·Journal of Neuroendocrinology·Vassilios PapadopoulosB Zirkin
Jul 7, 2019·Biochemical and Biophysical Research Communications·Xin YanManxiang Li
Jan 15, 2020·Seminars in Cancer Biology·Dongdong WangAtanas G Atanasov
May 16, 2021·Cellular Signalling·Yuliya FakhrDenise G Hemmings

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