Sphingosine 1-phosphate regulates IL-8 expression and secretion via S1PR1 and S1PR2 receptors-mediated signaling in extravillous trophoblast derived HTR-8/SVneo cells

Placenta
Daniela BrünnertPankaj Goyal

Abstract

Both villous and extravillous trophoblast (EVT) cells produce a wide range of cytokines and also respond to them in autocrine and paracrine manner. Deregulation of cytokine secretion may lead to various pathologic conditions including preeclampsia. IL-8, a pro-inflammatory cytokine, regulates various cellular functions such as neutrophil trafficking, cell adhesion, tumor growth and has a role in placental development. IL-8 also promotes trophoblast cell migration and invasion, and stimulates the secretion of progesterone. The induction and mechanism of IL-8 secretion by EVT is still unknown. IL-8 mRNA expression and secretion was determined using real-time PCR and ELISA respectively. To identify the mechanism of IL-8 expression and secretion, selective antagonists and agonist of S1P receptor subtypes, Rac1 and Rho-kinase inhibitors were used. We found that S1P induces IL-8 gene expression and protein secretion in EVT derived HTR-8/SVneo cells but not in BeWo cells. SEW2781, the selective agonist of S1PR(1), induced IL-8 gene expression but not protein secretion. The specific S1PR(2) inhibitor JTE-013 could drastically inhibit IL-8 secretion. Furthermore, pre-treatment of cells with the selective S1PR(1)/S1PR(3) antagonist VPC23...Continue Reading

References

Dec 11, 1992·Science·A E KochR M Strieter
Oct 1, 1992·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C H Graham, P K Lala
Aug 15, 1998·American Journal of Obstetrics and Gynecology·C L ElliottA A Calder
Jan 14, 1999·Human Reproduction·F C DenisonS C Riley
Jan 6, 2000·American Journal of Reproductive Immunology : AJRI·Y WangE Brown
May 2, 2001·Gynecologic Oncology·B M SchwartzY Xu
Jun 1, 2002·The Journal of Biological Chemistry·Rhett J CummingsViswanathan Natarajan
Oct 28, 2004·Thrombosis Research·Neil M GudeRoger G King
Oct 2, 2007·Trends in Endocrinology and Metabolism : TEM·Sergio E AlvarezSarah Spiegel
Aug 7, 2009·Reproductive Sciences·L K HarrisP N Baker
Mar 3, 2010·The Journal of Experimental Medicine·Carole A OskeritzianSarah Spiegel
May 6, 2010·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Miğraci TosunErdal Malatyalioğlu
May 7, 2011·Nature Reviews. Immunology·Sarah Spiegel, Sheldon Milstien
Oct 18, 2011·Trends in Cell Biology·Michael MaceykaSarah Spiegel
Aug 21, 2013·Nature Reviews. Drug Discovery·Gregory T KunkelSarah Spiegel

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