PMID: 11917049Mar 28, 2002Paper

Sphingosine 1-phosphate stimulates rat mesangial cell proliferation from outside the cells

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Norio HanafusaMasafumi Fukagawa

Abstract

Proliferation of mesangial cells (MCs) is the initial step in glomerulonephritis, and platelet-derived mediators have been shown to play a significant role in this proliferation. Sphingosine 1-phosphate (S1P), one of the sphingolipids, is abundantly stored in platelets and is released upon stimulation. We examined the effects of S1P and related sphingolipids on the cell fate of cultured MCs in order to elucidate potential roles of these lipid mediators in glomerulonephritis. Cell proliferation was evaluated by bromodeoxy uridine (BrdU) incorporation together with MTS assay. Apoptosis of MCs was evaluated by examining annexin V staining and typical morphological changes in nuclei. We also examined the metabolism of [(3)H]sphingosine in MCs in either the presence or absence of platelet-derived growth factor (PDGF). The expression of endothelial differentiation genes (edg), which are the cell surface receptors for S1P in MCs, was examined by RT-PCR. S1P, but not the other sphingolipids, stimulated MC proliferation. In contrast, dimethylsphingosine (DMS) induced apoptosis in the MCs. The amount of sphingosine (Sph) converted into S1P was small and was not affected by PDGF. This observation suggested that Sph kinase activity produci...Continue Reading

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Citations

Dec 18, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Susumu KatsumaGozoh Tsujimoto
Dec 17, 2008·American Journal of Physiology. Renal Physiology·R Jason KirbyLois J Arend
Oct 1, 2009·Kidney International·David A Long, Karen L Price
Mar 18, 2010·Biochemical and Biophysical Research Communications·Seiichiro AndoYoshinari Takasaki
Mar 6, 2009·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Toshiyuki ImasawaYutaka Yatomi
Feb 12, 2011·British Journal of Pharmacology·Mirjam SchuchardtMarkus van der Giet
Jun 25, 2013·Basic & Clinical Pharmacology & Toxicology·Stephanie SchwalmAndrea Huwiler
Oct 29, 2002·FEBS Letters·Shawn G PayneSarah Spiegel
Aug 30, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Tetsuhiro TanakaMasaomi Nangaku
Jan 11, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Makoto KuranoYutaka Yatomi
Dec 31, 2020·Journal of the American Society of Nephrology : JASN·Yelena DrexlerSandra Merscher

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis