PMID: 8955554Nov 1, 1996Paper

Sphingosine-induced inhibition of capacitative calcium influx in CFPAC-1 cells

Cell Calcium
S OrlatiM Rugolo

Abstract

Sphingosine (10 microM) induced mobilization of intracellular Ca2+ stores in the pancreatic duct adenocarcinoma cell line CFPAC-1. The effect was specific for sphingosine, since the sphingosine analog C2-ceramide had no effect. Sphingosine did not cause Ca2+ entry from extracellular medium, as also shown by following Mn2+ quenching of Fura-2 fluorescence. Furthermore, sphingosine, similarly to the mitochondrial inhibitors rotenone and oligomycin, strongly inhibited the rate of Mn2+ entry triggered by both thapsigargin- and agonist-induced depletion of intracellular stores. The uptake of rhodamine 123, a lipophilic cation which estimates mitochondrial energy level, was reduced by sphingosine to an extent similar to that observed in the presence of mitochondrial inhibitors. It is suggested that impairment of mitochondrial function might be responsible for inhibition of capacitative Ca2+ entry caused by sphingosine.

References

Aug 6, 1991·Biochemistry·R S Arnold, A C Newton
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R A SchoumacherT A Rado
Jul 29, 1991·FEBS Letters·H Sugiya, S Furuyama
Nov 11, 1990·Cell Calcium·J W Putney
Feb 1, 1991·Journal of Bioenergetics and Biomembranes·A H Merrill
Jul 1, 1991·The Journal of Cell Biology·H ZhangS Spiegel
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Nov 15, 1995·The Biochemical Journal·M J Berridge
Oct 1, 1994·Pflügers Archiv : European journal of physiology·S SchumannJ Leipziger
Feb 17, 1995·The Journal of Biological Chemistry·K WongN Hunchuk
Apr 1, 1994·Pflügers Archiv : European journal of physiology·L J GaliettaG Romeo
Jun 1, 1993·Cell Calcium·K Wong, L Kwan-Yeung
Jan 28, 1993·Nature·M J Berridge

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Citations

Sep 17, 1998·The Journal of Biological Chemistry·C MathesR Penner
Oct 12, 1999·The American Journal of Physiology·E KobrinskyA R Marks
Jan 17, 2003·The Journal of Biological Chemistry·Roland MalliWolfgang F Graier

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