Sphingosine inhibits voltage-operated calcium channels in GH4C1 cells.

The Journal of Biological Chemistry
A TitievskyKid Törnquist

Abstract

In the present study we investigated the mechanism of inhibitory action of sphingosine (SP) on voltage-activated calcium channels (VOCCs) in pituitary GH4C1 cells. Using the patch-clamp technique in the whole-cell mode, we show that SP inhibits Ba2+ currents (IBa) when 0.1 mM BAPTA is included in the patch pipette. However, when the BAPTA concentration was raised to 1-10 mM, SP was without a significant effect. The effect of SP was apparently not mediated via a kinase, as it was not inhibited by staurosporine. By using the double-pulse protocol (to release possible functional inhibition of the VOCCs by G proteins), we observed that G proteins apparently evoked very little functional inhibition of the VOCCs. Furthermore, including GDPbetaS (guanyl-5'-yl thiophosphate) in the patch pipette did not alter the inhibitory effect of SP on the Ba2+ current, suggesting that SP did not modulate the VOCCs via a G protein-dependent pathway. Single-channel experiments with SP in the pipette, and experiments with excised outside-out patches, suggested that SP directly inhibited VOCCs. The main mechanism of action was a dose-dependent prolongation of the closed time of the channels. The results thus show that SP is a potent inhibitor of VOCCs...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·A H Tashjian
May 1, 1990·Molecular Endocrinology·R N Day, R A Maurer
Jan 1, 1986·The Journal of General Physiology·D R Matteson, C M Armstrong
Jan 1, 1988·The American Journal of Physiology·C Marchetti, A M Brown
Jan 1, 1988·Vitamins and Hormones·H Glossmann, J Striessnig
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Dec 1, 1994·Trends in Neurosciences·B Hille
Feb 1, 1994·Journal of Molecular and Cellular Cardiology·C A DettbarnR A Sabbadini
Apr 1, 1994·The Biochemical Journal·K Törnquist, E Ekokoski
Jun 1, 1993·Cell Calcium·K Wong, L Kwan-Yeung
Jan 26, 1996·The Journal of Biological Chemistry·C van KoppenL Pott
Feb 1, 1996·The American Journal of Physiology·K Yasui, P Palade

❮ Previous
Next ❯

Citations

Mar 10, 2001·European Journal of Pharmacology·J SummanenH Vuorela
Oct 24, 2002·European Journal of Pharmacology·Kid TörnquistMichael Pasternack
Nov 21, 2007·Journal of Lipid Research·Nora TahallahOlivier Laprévote
Jan 27, 2010·Journal of Lipid Research·Nora P RotsteinO Lorena German
Apr 12, 2008·Prostaglandins & Other Lipid Mediators·Ana Olivera
Dec 2, 2004·Brain Research. Brain Research Reviews·Laura Colombaioni, Mercedes Garcia-Gil
Apr 8, 2009·Biophysical Journal·Hirotaka SasakiStephen H White
Jun 11, 2015·Journal of Cellular Physiology·Jarmila LakomáMarco Caprini
Feb 12, 2014·Biochemical and Biophysical Research Communications·Megumi SakumaNaoaki Saito
Feb 11, 2015·Biochimica Et Biophysica Acta·Frédérique SabourdyThierry Levade
Jun 25, 2013·Molecular and Cellular Endocrinology·Ajda FlaškerRobert Zorec
May 11, 2012·Cell Calcium·Jernej JorgačevskiRobert Zorec
Feb 17, 2017·Journal of Lipid Research·Blake R HopiavuoriRobert E Anderson
Nov 20, 2004·Molecular Pharmacology·Christian GrimmChristian Harteneck
Mar 14, 2007·Journal of Neurophysiology·Lieju LiuS A Simon
Jun 1, 2000·Biochimica Et Biophysica Acta·A HuwilerK Sandhoff
Oct 28, 2004·European Journal of Cell Biology·Nils BäckBetty A Eipper

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.