Sphingosine kinase 1 is critically involved in nitric oxide-mediated human endothelial cell migration and tube formation.

British Journal of Pharmacology
Stephanie SchwalmA Huwiler

Abstract

Sphingosine kinases (SKs) convert sphingosine to sphingosine 1-phosphate (S1P), which is a bioactive lipid that regulates a variety of cellular processes including proliferation, differentiation and migration. We used the human endothelial cell line EA.hy926 to investigate the effect of nitric oxide (NO) donors on SK-1 expression, and on cell migration and tube formation. We showed that exposure of EA.hy926 cells to Deta-NO (125-1000 microM) resulted in a time- and concentration-dependent up-regulation of SK-1 mRNA and protein expression, and activity with a first significant effect at 250 microM of Deta-NO. The increased SK-1 mRNA expression resulted from an enhanced SK-1 promoter activity. A similar effect was also seen with various other NO donors. In mechanistic terms, the NO-triggered effect occurred independently of cGMP, but involved the classical mitogen-activated protein kinase cascade because the MEK inhibitor U0126 abolished the NO-induced SK-1 expression. The effect of NO was also markedly reduced by the thiol-reducing agent N-acetylcysteine, suggesting a redox-dependent mechanism. Functionally, Deta-NO triggered an increase in the migration of endothelial cells in an adapted Boyden chamber assay, and also increased...Continue Reading

References

Jan 3, 1991·The New England Journal of Medicine·N WeidnerJ Folkman
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C J EdgellJ B Graham
Dec 30, 1993·The New England Journal of Medicine·S Moncada, A Higgs
Apr 1, 1993·British Journal of Pharmacology·E Pipili-SynetosM E Maragoudakis
Mar 31, 1995·The Journal of Biological Chemistry·H M LanderA Novogrodsky
Nov 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C EstradaC González
Aug 16, 1996·The Journal of Biological Chemistry·H M LanderJ M Tauras
Jan 17, 1997·The Journal of Biological Chemistry·E NoiriM S Goligorsky
Jul 9, 1997·Biochemical and Biophysical Research Communications·V J VenemaR C Venema
Feb 12, 1998·The American Journal of Physiology·E NoiriM S Goligorsky
Mar 7, 1998·British Journal of Pharmacology·S P OlesenA Mülsch
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·H Y YunT M Dawson
Jul 11, 1998·The Journal of Biological Chemistry·M F FavataJ M Trzaskos
Aug 26, 1998·Clinical and Experimental Immunology·K D KrönckeV Kolb-Bachofen
Mar 19, 1999·Clinical and Experimental Pharmacology & Physiology·M S GoligorskyE Noiri
May 14, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·T MuroharaJ M Isner
Jan 8, 2000·Clinical and Experimental Pharmacology & Physiology·P C LeeT R Billiar
Aug 21, 2001·Pflügers Archiv : European journal of physiology·J PfeilschifterK F Beck
May 16, 2002·The Journal of Biological Chemistry·Sarah Spiegel, Sheldon Milstien
Jun 19, 2002·Biochimica Et Biophysica Acta·Tracee Scalise Panetti
Nov 26, 2002·Biochemical and Biophysical Research Communications·Makoto OsadaYukio Ozaki
Jan 4, 2003·Hypertension·Katsuya AmanoToshiji Iwasaka
Jan 20, 2004·Biochimica Et Biophysica Acta·Yusuke NakadeTakashi Murate
Jan 28, 2004·Cell·Keith Burridge, Krister Wennerberg
Sep 1, 2004·Cellular and Molecular Life Sciences : CMLS·R Muñoz-ChápuliM Angel Medina
Feb 18, 2005·Acta Pharmacologica Sinica·Jian-dong Luo, Alex F Chen
Jun 14, 2005·The Journal of Biological Chemistry·Nitai C HaitSarah Spiegel
Jan 24, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Regina AlemanyDagmar Meyer Zu Heringdorf
Feb 12, 2008·Biochemical and Biophysical Research Communications·Stephanie SchwalmAndrea Huwiler
May 2, 2008·British Journal of Pharmacology·S P H AlexanderJ A Peters
Oct 9, 2008·Journal of Cardiovascular Pharmacology·Thomas LargiadèrThomas F Lüscher

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Citations

Dec 5, 2013·The Journal of Endocrinology·Pablo H CutiniVirginia L Massheimer
Jul 27, 2012·British Journal of Pharmacology·Bo WuRui Wang
Jan 21, 2011·Plant Signaling & Behavior·Isabelle GuillasEmmanuel Baudouin
Nov 25, 2011·Molecular and Cellular Biochemistry·Xing LiuGuang-Hui Yi
Sep 30, 2021·Biological Reviews of the Cambridge Philosophical Society·Mengqiao CuiHongjie Zhang

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