SPI-C and STAT6 can cooperate to stimulate IgE germline transcription

Biochemical and Biophysical Research Communications
Robert CarlssonT Leanderson

Abstract

SPI-C is a novel ETS protein that is expressed in B lymphocytes. No target gene for SPI-C has so far been defined. We have performed a yeast two-hybrid screen using SPI-C as bait in order to further analyze the functional role of this orphan transcription factor. We found that SPI-C interacted specifically with the C-terminus of STAT6 in yeast. By co-immunoprecipitation in transfected COS7 cells the physical interaction between SPI-C and STAT6 was confirmed. Furthermore, this protein-protein interaction is functional since we could demonstrate that SPI-C and STAT6 stimulated IL4 induced Iepsilon transcription synergistically but only when both proteins bound to DNA. Thus, a protein interaction between SPI-C and STAT6 is the basis for a novel mechanism for regulation of IL4 induced gene expression.

References

Jan 1, 1996·Molecular and Cellular Biology·M J Klemsz, R A Maki
Apr 3, 1999·The Journal of Biological Chemistry·M BemarkT Leanderson
Oct 16, 2003·Molecular and Cellular Biology·Natasha RekhtmanArthur I Skoultchi
Nov 1, 2003·Biochemical and Biophysical Research Communications·Satoshi HiroseHitoshi Sakano

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Citations

Feb 12, 2008·BMC Genomics·Bayram EdemirEberhard Schlatter
Sep 12, 2008·Genes and Immunity·I DebnathJ H Weis
Mar 15, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stephen K H LiRodney P DeKoter
Apr 29, 2020·Experimental & Molecular Medicine·Eun Mi GoNa Kyung Lee

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