Spiclomazine displays a preferential anti-tumor activity in mutant KRas -driven pancreatic cancer

Oncotarget
Xiaoyu GuoJin Wang

Abstract

Ras-targeted therapy represents a 'holy grail' in oncology. Based on our model prediction, Spiclomazine freezing the intermediate conformation of activated Ras is central to cancer therapeutics. We show here that Spiclomazine leads to an effective suppression in Ras-mediated signaling through abrogating the KRas-GTP level in the KRas-driven pancreatic cancer. The Ras-mediated signaling inhibition leads to dramatically reduced survivals of five KRas-driven pancreatic cancer cell lines with IC50 ranging 19.7~74.2 μM after 48 hours of treatment. However, no significant changes have been observed for normal cell lines. It is worth mentioning that the mutant KRas-driven cancer cells are more sensitive towards Spiclomazine than the wild-type KRas cancer cells. Subsequent cellular thermal shift and RNA interference assays show that Spiclomazine efficiently binds with and stabilizes KRas to a certain extent within the cells. This validates the effect of target engagement on drug efficacy. Furthermore, Spiclomazine arrests cell cycle at G2 phase in the cancer cells, without obvious cell-cycle arrest in the normal cells. This further demonstrates its selectively biological response to cancer cells involved in Ras-GTP-mediated target enga...Continue Reading

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Citations

Aug 21, 2021·ACS Medicinal Chemistry Letters·Jonathan D MortisonSimon M Bushell
Nov 10, 2021·Reports on Progress in Physics·Wen-Ting ChuJin Wang

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Methods Mentioned

BETA
thermal shift
xenograft
pulldown
pull-down
PCR
transfection
flow cytometry

Software Mentioned

SPSS

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