Spider minor ampullate silk proteins contain new repetitive sequences and highly conserved non-silk-like "spacer regions"

Protein Science : a Publication of the Protein Society
M A Colgin, Randolph Lewis

Abstract

Spider minor ampullate silk is a strong non-elastic deformably stretchable silk used in web formation. This silk from Nephila clavipes is composed of two proteins, MiSp 1 and 2, whose transcripts are 9.5 and 7.5 kb, respectively, as determined by Northern blots. Both MiSp proteins are organized into a predominantly repetitive region and a small nonrepetitive carboxy terminal region. These highly repetitive regions are composed mainly of glycine and alanine, but also contain tyrosine, glutamine, and arginine. The sequences are mainly GGX and GA repeats. The repetitive regions are interrupted by nonrepetitive serine-rich spacer regions. Although the sequences of the spacer regions differ from the repetitive regions, sequences of the spacers from different regions of the proteins are nearly identical. The sequence differences between major and minor ampullate silks may explain the differing mechanical properties of the fibers.

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Citations

Aug 9, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C Y Hayashi, R V Lewis
Dec 9, 2003·Journal of Molecular Biology·Elisabetta BiniDavid L Kaplan
Oct 9, 2001·International Journal of Biological Macromolecules·C Riekel, F Vollrath
Aug 16, 2000·Trends in Biotechnology·M B HinmanR V Lewis
Jul 27, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·B BochicchioA M Tamburro
Jan 5, 2002·Journal of Biotechnology·S Winkler, D L Kaplan
Jan 5, 2002·Journal of Biotechnology·K H GührsF Grosse
Jan 5, 2002·Journal of Biotechnology·S R FahnestockL A Bedzyk
Oct 20, 2010·Biomacromolecules·David J PerryRandolph V Lewis
Aug 29, 2012·The Journal of Biological Chemistry·Keshav VasanthavadaCraig Vierra
Aug 11, 2010·BMC Evolutionary Biology·Jessica E GarbCheryl Y Hayashi
Sep 29, 2011·BMC Biotechnology·Annika SciorElke Deuerling
Oct 4, 2007·PloS One·Alexander SponnerKlaus Weisshart
Feb 22, 2012·International Journal of Nanomedicine·Lijuan Sun, Xiaojun Zhao
Jan 21, 2011·Journal of Visualized Experiments : JoVE·Felicia JefferyCraig Vierra
Aug 22, 2013·Acta Biomaterialia·Olena TokarevaDavid L Kaplan
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May 8, 2014·Nature Communications·Kristian W SanggaardJun Wang

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