Spiegelmer-Based Sandwich Assay for Cardiac Troponin I Detection.

International Journal of Molecular Sciences
Zoltán János TolnaiTamás Mészáros

Abstract

Two subunits of the ternary troponin complex, I and C, have cardiac muscle specific isoforms, and hence could be applied as highly-selective markers of acute coronary syndrome. We aimed at paving the way for the development of a robust cardiac troponin I-detecting sandwich assay by replacing antibodies with nuclease resistant aptamer analogues, so-called spiegelmers. To complement the previously generated spiegelmers that were specific for the N-terminus of cTnI, spiegelmers were selected for an amino acid stretch in the proximity of the C-terminal part of the protein by using a D-amino acid composed peptide. Following the selection, the oligonucleotides were screened by filter binding assay, and surface plasmon resonance analysis of the most auspicious candidates demonstrated that this approach could provide spiegelmers with subnanomolar dissociation constant. To demonstrate if the selected spiegelmers are functional and suitable for cTnI detection in a sandwich type arrangement, AlphaLisa technology was leveraged and the obtained results demonstrated that spiegelmers with different epitope selectivity are suitable for specific detection of cTnI protein even in human plasma containing samples. These results suggest that spiege...Continue Reading

References

Dec 1, 1989·Journal of Molecular and Cellular Cardiology·H A KatusW Kübler
Sep 1, 1996·Nature Biotechnology·S KlussmannJ P Fürste
Sep 20, 2000·Accounts of Chemical Research·M FamulokM Blind
Jun 12, 2003·Nucleic Acids Research·Werner G PurschkeSven Klussmann
Aug 18, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Masayasu Kuwahara, Naoki Sugimoto
May 19, 2011·Angewandte Chemie·Jens MüllerBernd Pötzsch
Aug 23, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mark S LowenthalDavid M Bunk
Jul 28, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kai HoehligSven Klussmann
Jan 8, 2014·Methods in Molecular Biology·Szilvia K Nagy, Tamás Mészáros
May 20, 2014·Chemical Communications : Chem Comm·Zsuzsanna SzeitnerTamás Mészáros
Jun 1, 2014·Journal of Pharmaceutical and Biomedical Analysis·Zsuzsanna SzeitnerTamás Mészáros
Nov 23, 2015·Chemistry & Biology·Charles OleaGerald F Joyce
Feb 27, 2016·Current Opinion in Structural Biology·Amy D GelinasNebojsa Janjic
Feb 12, 2017·Internal and Emergency Medicine·Pankaj GargPhilip Haaf
Oct 2, 2019·Expert Review of Cardiovascular Therapy·Gerald FitzgeraldThomas J Kiernan

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Citations

Aug 17, 2020·International Journal of Molecular Sciences·Zsófia Bognár, Róbert E Gyurcsányi

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Methods Mentioned

BETA
enzyme capture assay
SELEX
EXponential Enrichment
ELISA
PCR
surface plasmon resonance
filter binding
chip
AlphaLISA
electrophoresis

Software Mentioned

BiaLogic
Scrubber
AlphaLisa

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