Spike mutation D614G alters SARS-CoV-2 fitness and neutralization susceptibility

BioRxiv : the Preprint Server for Biology
Jessica A PlantePei-Yong Shi


A spike protein mutation D614G became dominant in SARS-CoV-2 during the COVID-19 pandemic. However, the mutational impact on viral spread and vaccine efficacy remains to be defined. Here we engineer the D614G mutation in the SARS-CoV-2 USA-WA1/2020 strain and characterize its effect on viral replication, pathogenesis, and antibody neutralization. The D614G mutation significantly enhances SARS-CoV-2 replication on human lung epithelial cells and primary human airway tissues, through an improved infectivity of virions with the spike receptor-binding domain in an "up" conformation for binding to ACE2 receptor. Hamsters infected with D614 or G614 variants developed similar levels of weight loss. However, the G614 virus produced higher infectious titers in the nasal washes and trachea, but not lungs, than the D614 virus. The hamster results confirm clinical evidence that the D614G mutation enhances viral loads in the upper respiratory tract of COVID-19 patients and may increases transmission. For antibody neutralization, sera from D614 virus-infected hamsters consistently exhibit higher neutralization titers against G614 virus than those against D614 virus, indicating that (i) the mutation may not reduce the ability of vaccines in c...Continue Reading


Sep 10, 2020·Nature·Ewen Callaway
Jan 6, 2021·Journal of Applied Genetics·Szymon HryhorowiczEwa Ziętkiewicz
Nov 6, 2020·Biomédica : revista del Instituto Nacional de Salud·Francisco J DíazMaría Teresa Rugeles
Apr 8, 2021·Emerging Microbes & Infections·Marco BrustolinJoaquim Segalés
Mar 7, 2021·Molecular Biology and Evolution·Maloyjo Joyraj BhattacharjeeWen-Hsiung Li
Apr 27, 2021·Frontiers in Cellular Neuroscience·Petra TavčarJernej Jorgačevski
Apr 30, 2021·Scientific Reports·Mohamed Raef Smaoui, Hamdi Yahyaoui
Jun 10, 2021·Computational and Structural Biotechnology Journal·L Ponoop Prasad PatroThenmalarchelvi Rathinavelan

Methods Mentioned


Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.