PMID: 8958135Jan 1, 1997Paper

Spin label oximetry to assess extracellular oxygen during myocardial ischemia

Free Radical Biology & Medicine
J E BakerB Kalyanaraman

Abstract

We describe real-time measurement of myocardial oxygen consumption during ischemia in the intact heart. Measurement of extracellular oxygen concentration during myocardial ischemia by spin label oximetry has been limited by ischemia-induced reduction of the neutral, water-soluble nitroxide TEMPONE. We have overcome this problem by encapsulating the nitroxides. Isolated immature (7-10 d old) rabbit hearts (n = 8) were perfused aerobically within the cavity of a loop gap resonator with bicarbonate buffer containing an oxygen-sensitive, lipid-soluble nitroxide (14N-TEMPO laurate in FC-43 perfluorocarbon micelles) and a much less oxygen-sensitive and positively charged nitroxide (15N-TEMPO choline in multilamellar vesicles) as an internal standard. The ratio of the ESR signal amplitudes of these nitroxides was used as a sensitive index of oxygen concentration. Sequestration of the nitroxides decreased their reduction rate by ascorbate in comparison with nonsequestered nitroxides. Hearts were subjected to 60 min of global no-flow ischemia at 20 degrees C. Extracellular oxygen content (mean +/- SD) during aerobic perfusion was 1195 +/- 55 mumol/liter. The electron spin resonance signal from TEMPO laurate increased with the onset and ...Continue Reading

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Citations

Jan 18, 2003·Journal of Pharmaceutical Sciences·Keita SaitoToshihiko Ozawa
Jan 30, 2007·Biophysical Journal·Anna BrataszPeriannan Kuppusamy
Dec 14, 2011·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Denis A KomarovValery V Khramtsov
Jan 9, 2010·The Journal of Physical Chemistry. B·I KirilyukE Bagryanskaya

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