Spin trapping studies of alcohol-initiated radicals in rat liver: influence of dietary fat

The Journal of Nutrition
L A Reinke, P B McCay

Abstract

We conducted spin trapping experiments to test the effects of acute and chronic alcohol consumption in livers from rats that had been fed either high fat (35% of energy) or low fat (12% of energy) liquid diets. Rats were anesthetized with isoflurane, the spin trapping agent POBN [alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone] was administered by intravenous injection, and bile samples were collected for electron paramagnetic resonance (EPR) analyses. Two different types of EPR spectra were observed in bile from the animals in these studies. One set of spectral lines was from the 1-hydroxyethyl radical adduct of POBN, which was conclusively identified by injecting the rats with [1-13C]ethanol. The EPR signals of a second type of radical adduct in bile could be observed both before and after acute administration of ethanol. Although the radical(s) responsible for this second series of signals could not be conclusively identified, it is likely that lipid radicals were formed under these conditions and trapped by POBN. For both types of radical adducts, the most intense EPR signals were observed in rats that had been fed alcohol in combination with a high fat diet for 2 wk before the experiments. These results confirm and extend previ...Continue Reading

References

Jan 1, 1992·Free Radical Biology & Medicine·R NordmannH Rouach
Jan 1, 1990·Free Radical Research Communications·L A ReinkeP B McCay
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L A ReinkeP B McCay
Jan 1, 1988·Chemico-biological Interactions·E AlbanoM U Dianzani
Sep 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H TsukamotoS W French
Mar 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H TsukamotoC Largman
Jan 1, 1982·Alcoholism, Clinical and Experimental Research·C S Lieber, L M DeCarli
Jun 1, 1993·Archives of Biochemistry and Biophysics·K T Knecht, R P Mason

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Citations

Oct 2, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E Mezey
Jun 23, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shao-Yu ChenKathleen K Sulik
Mar 1, 2007·Indian Journal of Clinical Biochemistry : IJCB·G KumarM Rajasekara Pandian

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