PMID: 8951421Dec 1, 1996Paper

Spin trapping study in the lungs and liver of F344 rats after exposure to ozone

Free Radical Research
R VincentT M Bray

Abstract

Fischer 344 rats were injected with the spin traps C-phenyl N-tert-butyl nitrone (PBN, 150 mg/kg bw, ip) or 4-pyridine-N-oxide N-tert-butyl nitrone (POBN, 775 mg/kg bw, ip), and exposed to clean air or 2 ppm ozone for two hours. The presence of spin adducts was determined by electron paramagnetic resonance (EPR) spectroscopy of chloroform extracts of lung and liver homogenates. No significant levels of adducts were detected in the lungs of air control animals. Benzoyl N-tert-butyl aminoxyl, attributed to direct reaction of ozone with PBN, and tert-butyl hydroaminoxyl, the scission product of the hydroxyl adduct of PBN, were detected in the lungs of ozone exposed rats. EPR signals for carbon-centred alkoxyl and alkyl adducts were also detected with PBN in the lungs and liver of animals exposed to ozone. With POBN, only carbon-centred alkyl radicals were detected. Senescent, 24 months old rats were found to retain about twice more 14C-PBN in blood, heart and lungs by comparison to juvenile, 2 months old animals. Accordingly, the EPR signals were generally stronger in the lungs of the senescent rats by comparison to juvenile rats. Together, the observations were consistent with the previously proposed notion that a significant flu...Continue Reading

References

Jan 1, 1992·Free Radical Biology & Medicine·E G JanzenR D Hinton
Jan 1, 1991·Free Radical Biology & Medicine·W A Pryor, D F Church
Dec 1, 1994·Environmental Health Perspectives·D L LaskinJ D Laskin
Sep 1, 1994·American Journal of Respiratory and Critical Care Medicine·G E HatchJ McKee
Nov 1, 1996·Canadian Journal of Physiology and Pharmacology·L Liu, D L Severson

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Citations

Dec 31, 1997·American Journal of Respiratory and Critical Care Medicine·L LiuF S Silverman
Feb 24, 2007·Toxicology and Applied Pharmacology·Adrian C NicolescuThomas E Massey
Jan 6, 2000·Experimental Gerontology·E KoikeM Murakami
Jan 1, 2002·Toxicology Mechanisms and Methods·Prem KumarathasanJosée Guénette

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