Spin trapping study of antioxidant properties of neopterin and 7,8-dihydroneopterin

Biochemical and Biophysical Research Communications
K OettlG Reibnegger

Abstract

Previously, it has been shown that pteridine derivatives are capable of modulating the action of free radicals and both prooxidant and antioxidant properties have been described. However, the mechanism of manifestation of these properties is still unclear. We studied the radical scavenging properties of 7,8-dihydroneopterin and neopterin using the spin trap 5,5-dimethyl-1-pyrroline-1-oxide (DMPO). It was found that dihydroneopterin acts generally as a radical scavenger. In the presence of dihydroneopterin the ESR signal was reduced by 30 to 90% compared to the control signal. The rate constants for the reactions of 7,8-dihydroneopterin with superoxide (10(3) M(-1) s(-1)) and peroxyl radicals (10(7) M(-1) s(-1)) were determined. Neopterin in contrast showed no reduction of the ESR signal except with superoxide radicals produced by xanthine oxidase. However, this effect was shown to be due to an inhibition of enzyme rather than to radical scavenging. Our results provide a basis for understanding previous observations of radical scavenger activity of 7,8-dihydroneopterin.

Citations

Dec 22, 1998·Free Radical Research·I WedeD Fuchs
Sep 11, 2004·Biochemical and Biophysical Research Communications·Karl OettlGilbert Reibnegger
Aug 29, 2001·Redox Report : Communications in Free Radical Research·S DugganS P Gieseg
Aug 12, 2000·Redox Report : Communications in Free Radical Research·S P GiesegD Glubb
Aug 10, 2015·The International Journal of Biochemistry & Cell Biology·Hanadi KatouahSteven P Gieseg
Dec 10, 1999·Renal Failure·E N Wardle
Sep 28, 2004·The Journal of Biological Chemistry·Marcelo G BoniniOhara Augusto
Jun 29, 2018·Antioxidants·Steven P GiesegAngus Lindsay
Jul 24, 1998·Biochemical and Biophysical Research Communications·B WidnerD Fuchs
Oct 21, 1999·Biochemical and Biophysical Research Communications·K OettlG Reibnegger
Mar 20, 1999·Biochimica Et Biophysica Acta·K Oettl, G Reibnegger
Jul 22, 2008·Biochemical Pharmacology·Sergey DikalovJack L Arbiser

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