PMID: 8460133Mar 15, 1993Paper

Spinach thioredoxin m inhibits DNA synthesis in fertilized Xenopus eggs

Proceedings of the National Academy of Sciences of the United States of America
H HartmanJ C Gerhart

Abstract

A role for thioredoxin in metazoan DNA synthesis has been assessed by injecting rapidly dividing Xenopus eggs with purified heterologous thioredoxins, which might act as inhibitors if they were to replace resident thioredoxins in some but not all reaction steps. Of 10 tested proteins, spinach chloroplast thioredoxin m is the most potent inhibitor. Eggs cleave and produce cells lacking nuclei. DNA synthesis is severely reduced. Development arrests before gastrulation. In egg extracts, thioredoxin m inhibits incorporation of radioactive dCTP into DNA of sperm nuclei and M13 phage. Inhibition exceeds 90% when thioredoxin m and M13 DNA are preincubated together. The data support the interpretation that thioredoxins normally participate in initiation of metazoan DNA synthesis.

References

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Citations

Apr 28, 2004·Cytokine & Growth Factor Reviews·Dhananjaya V Kalvakolanu
Feb 4, 1998·Life Sciences·O Golubnitchaya-LabudovaG Lubec
Jul 23, 2009·Antioxidants & Redox Signaling·Michika MochizukiHiroshi Masutani
Feb 17, 2005·Molecular and Cellular Biology·Cemile JakupogluMarcus Conrad
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·H YanoB B Buchanan
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·N MouahebY Meyer
May 2, 2001·The Journal of Biological Chemistry·X MaD V Kalvakolanu
Aug 1, 1997·The New Phytologist·Jean-Pierre JacquotYves Meyer

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