Spinal activation of protein kinase C elicits phrenic motor facilitation

Respiratory Physiology & Neurobiology
Michael J Devinney, G S Mitchell

Abstract

The protein kinase C family regulates many cellular functions, including multiple forms of neuroplasticity. The novel PKCθ and atypical PKCζ isoforms have been implicated in distinct forms of spinal, respiratory motor plasticity, including phrenic motor facilitation (pMF) following acute intermittent hypoxia or inactivity, respectively. Although these PKC isoforms are critical in regulating spinal motor plasticity, other isoforms may be important for phrenic motor plasticity. We tested the impact of conventional/novel PKC activator, phorbol 12-myristate 13-acetate (PMA) on pMF. Rats given cervical intrathecal injections of PMA exhibited pMF, which was abolished by pretreatment of broad-spectrum PKC inhibitors bisindolymalemide 1 (BIS) or NPC-15437 (NPC). Because PMA fails to activate atypical PKC isoforms, and NPC does not block PKCθ, this finding demonstrates that classical/novel PKC isoforms besides PKCθ are sufficient to elicit pMF. These results advance our understanding of mechanisms producing respiratory motor plasticity, and may inspire new treatments for disorders that compromise breathing, such as ALS, spinal injury and obstructive sleep apnea.

Citations

Oct 16, 2018·Chest·Abdulghani SankariM Safwan Badr

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Michael J DevinneyG S Mitchell
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Francis J GolderG S Mitchell
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
K A StreyT L Baker-Herman
© 2022 Meta ULC. All rights reserved