Spinal neuronal cannabinoid receptors mediate urodynamic effects of systemic fatty acid amide hydrolase (FAAH) inhibition in rats

Neurourology and Urodynamics
Claudius FüllhasePetter Hedlund

Abstract

To test if urodynamic effects from systemic Fatty Acid Amide Hydrolase (FAAH) inhibition involve sacral spinal cannabinoid type 1 (CB1) or type 2 (CB2) receptors. Male rats with or without partial urethral obstruction were used for cystometry or immunohistochemistry. Urodynamic effects of intravenous (IV) 0.3 mg/kg Oleoyl Ethyl Amide (OEtA; FAAH inhibitor), and intrathecal (IT) 5 μg rimonabant (CB1 antagonist) or 5 μg SR144528 (CB2 antagonist) were studied in awake rats. After administration of rimonabant or SR144528, non-obstructed rats with normal bladder function developed bladder overactivity (BO), which was counteracted by OEtA. OEtA also counteracted BO in obstructed rats. SR144528 did not affect bladder function in obstructed rats but counteracted the urodynamic effects of OEtA. Surprisingly, rimonabant (and AM251, another CB1 antagonist) reduced BO in obstructed rats, whereafter OEtA produced no additional urodynamic effects. CB1 expression increased in the sacral spinal cord of obstructed rats whereas no changes were observed for CB2 or FAAH. Urodynamic effects of systemic FAAH inhibition involve activities at spinal neuronal CB1 and CB2 receptors in normal and obstructed rats. Endogenous spinal CB receptor ligands see...Continue Reading

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Dec 12, 2012·The Journal of Urology·Claudius FüllhasePetter Hedlund
Dec 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Carola Sales-CarbonellDavid Robbe
Aug 3, 2013·Neurourology and Urodynamics·Lysanne CampeauKarl-Erik Andersson
Sep 17, 2013·Neurourology and Urodynamics·Giorgio GandagliaPetter Hedlund
Sep 24, 2013·BJU International·Claudius FüllhaseKarl-Erik Andersson
Nov 5, 2013·The Journal of Urology·Zun-Yi WangDale E Bjorling

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Citations

Jul 6, 2016·Nature Reviews. Urology·Petter Hedlund, Christian Gratzke
Dec 9, 2016·American Journal of Physiology. Renal Physiology·Xuewen JiangChangfeng Tai

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