Spindle assembly checkpoint acquisition at the mid-blastula transition

PloS One
Maomao ZhangMichael A Lampson

Abstract

The spindle assembly checkpoint (SAC) maintains the fidelity of chromosome segregation during mitosis. Nonpathogenic cells lacking the SAC are typically only found in cleavage stage metazoan embryos, which do not acquire functional checkpoints until the mid-blastula transition (MBT). It is unclear how proper SAC function is acquired at the MBT, though several models exist. First, SAC acquisition could rely on transcriptional activity, which increases dramatically at the MBT. Embryogenesis prior to the MBT relies primarily on maternally loaded transcripts, and if SAC signaling components are not maternally supplied, the SAC would depend on zygotic transcription at the MBT. Second, checkpoint acquisition could depend on the Chk1 kinase, which is activated at the MBT to elongate cell cycles and is required for the SAC in somatic cells. Third, SAC function could depend on a threshold nuclear to cytoplasmic (N:C) ratio, which increases during pre-MBT cleavage cycles and dictates several MBT events like zygotic transcription and cell cycle remodeling. Finally, the SAC could by regulated by a timer mechanism that coincides with other MBT events but is independent of them. Using zebrafish embryos we show that SAC acquisition at the MBT...Continue Reading

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Citations

Mar 17, 2017·Molecular Biology of the Cell·Lydia R HeasleyJennifer G DeLuca
Oct 6, 2017·The Journal of Cell Biology·Simon I R Lane, Keith T Jones
Nov 8, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Cayetana Vázquez-Diez, Greg FitzHarris
Apr 25, 2018·Molecular Biology of the Cell·Abigail R GerholdPaul S Maddox
Mar 1, 2018·Molecular & Cellular Oncology·Ksenia KrupinaIzabela Sumara
Apr 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Shai Adar-LevorNatalie Elia
May 25, 2021·Frontiers in Cellular and Infection Microbiology·Caroline S SimonJulien Guizetti

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Methods Mentioned

BETA
in vitro transcription

Software Mentioned

Metamorph
ImageJ

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