Spindle checkpoint protein Xmad1 recruits Xmad2 to unattached kinetochores

The Journal of Cell Biology
R H ChenAndrew W Murray

Abstract

The spindle checkpoint prevents the metaphase to anaphase transition in cells containing defects in the mitotic spindle or in chromosome attachment to the spindle. When the checkpoint protein Xmad2 is depleted from Xenopus egg extracts, adding Xmad2 to its endogenous concentration fails to restore the checkpoint, suggesting that other checkpoint component(s) were depleted from the extract through their association with Xmad2. Mass spectrometry provided peptide sequences from an 85-kD protein that coimmunoprecipitates with Xmad2 from egg extracts. This information was used to clone XMAD1, which encodes a homologue of the budding yeast (Saccharomyces cerevisiae) checkpoint protein Mad1. Xmad1 is essential for establishing and maintaining the spindle checkpoint in egg extracts. Like Xmad2, Xmad1 localizes to the nuclear envelope and the nucleus during interphase, and to those kinetochores that are not bound to spindle microtubules during mitosis. Adding an anti-Xmad1 antibody to egg extracts inactivates the checkpoint and prevents Xmad2 from localizing to unbound kinetochores. In the presence of excess Xmad2, neither chromosomes nor Xmad1 are required to activate the spindle checkpoint, suggesting that the physiological role of Xm...Continue Reading

References

Mar 1, 1992·Molecular and Cellular Biology·R H ChenJ Blenis
Aug 9, 1991·Cell·R Li, Andrew W Murray
Jan 10, 1991·Nature·M GlotzerM W Kirschner
Nov 1, 1995·The Journal of Cell Biology·K G Hardwick, Andrew W Murray
Feb 16, 1995·Nature·X Li, R B Nicklas
Dec 1, 1994·Molecular and Cellular Biology·B T RobertsM A Hoyt
Jan 1, 1996·Analytical Chemistry·M Wilm, M Mann
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Oct 11, 1996·Science·Y Li, R Benezra
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·X HeShelley Sazer
Aug 22, 1997·Cell·M SchwabW Seufert
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y LiR Benezra
Jan 13, 1998·Current Opinion in Cell Biology·A Hershko
Jan 13, 1998·Current Opinion in Cell Biology·O Cohen-Fix, D Koshland
Feb 4, 1998·Trends in Genetics : TIG·K G Hardwick
Mar 7, 1998·Science·L H HwangAndrew W Murray
Apr 1, 1998·Nature·D P CahillB Vogelstein
May 5, 1998·Molecular and Cellular Biology·K A Farr, M A Hoyt
Jun 12, 1998·The Journal of Cell Biology·J C WatersE D Salmon
Jun 12, 1998·The Journal of Cell Biology·G J GorbskyAndrew W Murray

Citations

Apr 1, 2010·Chromosoma·André MaiaSusanne M A Lens
Mar 21, 2013·Chromosoma·Hironori Funabiki, David J Wynne
May 24, 2005·Gynecologic Oncology·Keun-Ho LeeJong-Sup Park
Jun 18, 2003·Biochemical and Biophysical Research Communications·Naihan XuDonald C Chang
Oct 22, 2003·Archives of Medical Research·C Lopes, Claudio Sunkel
Dec 11, 2002·Current Opinion in Cell Biology·Hongtao Yu
Dec 22, 1999·Current Opinion in Cell Biology·W Zachariae
Feb 19, 2000·Current Opinion in Genetics & Development·Daniel J Burke
Mar 31, 2000·Trends in Cell Biology·R D Gardner, Daniel J Burke
Apr 20, 2006·Nature Cell Biology·Daniel R FoltzDon W Cleveland
Oct 1, 2005·Nature Reviews. Cancer·Geert J P L KopsDon W Cleveland
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, E D Salmon
Feb 24, 2009·Nature Reviews. Molecular Cell Biology·Stephan GüttingerUlrike Kutay
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, K G Hardwick
Aug 3, 1999·European Journal of Biochemistry·K A Farr, O Cohen-Fix
Jul 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sean D RyanBeth A Weaver
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·R Li
May 15, 2002·Molecular Biology of the Cell·Eunah Chung, Rey-Huei Chen
Apr 11, 2003·Molecular Biology of the Cell·Song-Tao LiuTim J Yen
Oct 22, 2002·Molecular Biology of the Cell·Katie B ShannonE D Salmon
Jul 23, 2004·Molecular Biology of the Cell·S VigneronT Lorca
Feb 11, 2005·Molecular Biology of the Cell·Lisa A Hawryluk-GaraRichard Wozniak
Jun 20, 2008·Molecular Biology of the Cell·Katerina R KatsaniValérie Doye
May 20, 2011·Molecular Biology of the Cell·Erin L BarnhartScott C Schuyler
Oct 4, 2011·Molecular Biology of the Cell·Robert S HaganP K Sorger
Nov 29, 2002·The EMBO Journal·Toshiyuki HabuTomohiro Matsumoto
Dec 31, 2005·Biology of Reproduction·Marta Sikora-PolaczekMaria A Ciemerych
May 18, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anna DeAntoniAndrea Musacchio
Jul 19, 2001·Genes & Development·J Chen, Guowei Fang
May 19, 2011·Development, Growth & Differentiation·Yasuo Maeda
Aug 5, 2003·Genome Génome / Conseil National De Recherches Canada·Claudicéia Risso-PascottoCacilda Borges do Valle
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Larissa J VosG K Chan
Nov 22, 2011·Journal of Cell Science·Frederick G WesthorpeStephen S Taylor
May 8, 2009·Cell Structure and Function·Yujian LuHeng Liu
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·D A SkoufiasR L Margolis
Feb 13, 2001·Current Opinion in Genetics & Development·K Wassmann, R Benezra
Apr 8, 2010·Cellular and Molecular Life Sciences : CMLS·Richard WozniakValérie Doye
Jan 23, 2013·The Journal of Cell Biology·Stuart CaneThomas J Maresca
Sep 8, 2005·The Journal of Cell Biology·Yinghui MaoDon W Cleveland
Dec 24, 2003·The Journal of Cell Biology·Brian J TunquistJames L Maller
Apr 13, 2005·The Journal of Cell Biology·Geert J P L KopsDon W Cleveland
Mar 8, 2000·The Journal of Cell Biology·K G HardwickAndrew W Murray
Dec 15, 2010·The Journal of Cell Biology·Sang Hyun LeeHideyuki Saya
Nov 26, 2008·The Journal of Cell Biology·John J SkinnerBen E Black
Oct 17, 2007·The Journal of Cell Biology·Karthik JeganathanJan M van Deursen
Nov 4, 2015·The Journal of Cell Biology·Christian R NelsonNeedhi Bhalla
Aug 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Mayuko HaraXuelian Luo
Jan 8, 2008·Molecular and Cellular Endocrinology·Jacek Z KubiakMaria A Ciemerych
Dec 11, 2007·Biochimica Et Biophysica Acta·Barbara T McGroganAmanda McCann
Jan 6, 2006·The EMBO Journal·Stéphanie Miserey-LenkeiBruno Goud
Feb 21, 2009·The EMBO Journal·Mylavarapu V S SivaramStephen J Doxsey
Mar 10, 2006·The EMBO Journal·Marina MapelliAndrea Musacchio
Jul 21, 2011·The EMBO Journal·Luca L FavaAnna Santamaria
Nov 14, 2015·Cancer Chemotherapy and Pharmacology·Rustelle Janse van VuurenAnnie M Joubert
Sep 9, 2015·The Journal of Cell Biology·David J Wynne, Hironori Funabiki
May 30, 2015·Molecular Biology of the Cell·Nathaniel I KrefmanGeorjana Barnes
May 14, 1999·Current Opinion in Genetics & Development·K W DobieG H Karpen
Feb 23, 2005·Current Biology : CB·K G Hardwick
Feb 8, 2005·Current Biology : CB·Anna De AntoniAndrea Musacchio
Jun 9, 2004·Current Biology : CB·Bonnie J HowellE D Salmon
Apr 24, 2012·Current Biology : CB·Lindsey A ShepperdJonathan B A Millar
Jan 3, 2012·Current Biology : CB·Derek T C Lau, Andrew W Murray
May 12, 2005·Current Biology : CB·Eulalie BuffinRoger E Karess
May 1, 2007·Biochimica Et Biophysica Acta·Maggie K-L FungXianghong Wang
Mar 31, 2005·Cell·Kim Nasmyth
Oct 13, 2000·Cell·Ariane AbrieuDon W Cleveland
Nov 13, 2008·Structure·Xuelian Luo, Hongtao Yu
Nov 29, 2016·Annual Review of Genetics·Ryan M Naylor, Jan M van Deursen
Dec 30, 2003·Molecular & Cellular Proteomics : MCP·Bianca HabermannAndrej Shevchenko
Nov 6, 2015·Cells·Ikram Mossaid, Birthe Fahrenkrog
Jul 12, 2003·Nature Cell Biology·Eunah Chung, Rey-Huei Chen
Jul 16, 2005·BioTechniques·Jennifer I SempleDavid Markie
Nov 15, 2017·Cold Spring Harbor Symposia on Quantitative Biology·Pablo Lara-GonzalezArshad Desai
May 12, 2005·Cell Motility and the Cytoskeleton·Sheila Kadura, Shelley Sazer
Sep 5, 2001·Nature Reviews. Molecular Cell Biology·K Kitagawa, P Hieter
Jun 10, 2006·The Journal of Biological Chemistry·Sergiy I BorysovThomas M Guadagno
Sep 8, 2019·The Journal of Cell Biology·Jonathan Kuhn, Sophie Dumont
Dec 31, 2003·The Journal of Biological Chemistry·Rajnish BharadwajHongtao Yu
Jan 9, 2020·The Journal of Cell Biology·Sofia Cunha-SilvaCarlos Conde
Jan 15, 2020·The Journal of Cell Biology·Arnaud RondeletAlexander W Bird
Dec 1, 2001·The Journal of Biological Chemistry·Takefumi KasaiKuan-Teh Jeang
Aug 12, 2003·Oncogene·Michael L GatzaSusan J Marriott
Mar 17, 2004·Oncogene·Rajnish Bharadwaj, Hongtao Yu
May 19, 2020·Cell Cycle·Prabha SarangiAlan D D'Andrea
Jul 13, 2000·Cell Motility and the Cytoskeleton·M S CampbellG J Gorbsky
Jul 23, 2020·Molecular Biology of the Cell·Lénaïg DéfachellesNeedhi Bhalla
May 27, 2017·Proteomics·Zeynep Cansu Üretmen KagıalıNurhan Özlü
Jun 6, 2018·Frontiers in Cell and Developmental Biology·Yibo LuoSong-Tao Liu
Apr 3, 2020·The Journal of Cell Biology·Mark JackmanJonathon Pines
Jul 11, 2019·Nucleic Acids Research·Fengjie YuanYing Zhao
Apr 6, 2019·Nature Communications·Jun WanBeth A Weaver
Mar 11, 2020·Frontiers in Genetics·Xiang DingHesheng Luo
Apr 3, 2004·Cancer Research·Tamotsu SudoNaoto T Ueno

Related Concepts

AF126481
Xmad1 protein, Xenopus
SMAD2 protein, human
Smad2 protein, Xenopus
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