Spindle pole body duplication in fission yeast occurs at the G1/S boundary but maturation is blocked until exit from S by an event downstream of cdc10+

Molecular Biology of the Cell
Satoru UzawaW Zacheus Cande

Abstract

The regulation and timing of spindle pole body (SPB) duplication and maturation in fission yeast was examined by transmission electron microscopy. When cells are arrested at G1 by nitrogen starvation, the SPB is unduplicated. On release from G1, the SPBs were duplicated after 1-2 h. In cells arrested at S by hydroxyurea, SPBs are duplicated but not mature. In G1 arrest/release experiments with cdc2.33 cells at the restrictive temperature, SPBs remained single, whereas in cells at the permissive temperature, SPBs were duplicated. In cdc10 mutant cells, the SPBs seem not only to be duplicated but also to undergo partial maturation, including invagination of the nuclear envelope underneath the SPB. There may be an S-phase-specific inhibitor of SPB maturation whose expression is under control of cdc10(+). This model was examined by induction of overreplication of the genome by overexpression of rum1p or cdc18p. In cdc18p-overexpressing cells, the SPBs are duplicated but not mature, suggesting that cdc18p is one component of this feedback mechanism. In contrast, cells overexpressing rum1p have large, deformed SPBs accompanied by other features of maturation and duplication. We propose a feedback mechanism for maturation of the SPB t...Continue Reading

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Citations

Dec 26, 2009·Journal of Neuro-oncology·Zhi-fan JiaPei-yu Pu
Apr 6, 2007·Molecular Biology of the Cell·Ekaterina L GrishchukJ Richard McIntosh
Oct 23, 2010·PLoS Biology·Stefania CastagnettiPaul Nurse
Jun 23, 2015·FEBS Letters·Takeshi MizuguchiShiv I S Grewal
Oct 26, 2007·Journal of Cellular Physiology·Thomas M DurcanEdward H Hinchcliffe
Oct 13, 2006·The EMBO Journal·Ekaterina L Grishchuk, J Richard McIntosh
Mar 6, 2007·Developmental Cell·Johanna L HöögClaude Antony
Feb 20, 2007·Current Biology : CB·Sven K VogelIva Tolić-Nørrelykke
Apr 5, 2007·Current Biology : CB·Steven B Haase, Daniel J Lew
Jun 10, 2008·Bioscience, Biotechnology, and Biochemistry·Koei Okazaki, Osami Niwa
Sep 22, 2017·Annual Review of Genetics·Ann M Cavanaugh, Sue L Jaspersen
Mar 10, 2016·Molecular and Cellular Biology·Diana Rüthnick, Elmar Schiebel
Jun 18, 2017·The Journal of Cell Biology·Andrew J BestulSue L Jaspersen
Jul 12, 2005·Yeast
Oct 29, 2020·Current Opinion in Structural Biology·Sue L Jaspersen
May 23, 2008·Journal of Cell Science·Rafael R Daga, Paul Nurse

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