SPINKL, a Kazal-type serine protease inhibitor-like protein purified from mouse seminal vesicle fluid, is able to inhibit sperm capacitation

Reproduction : the Official Journal of the Society for the Study of Fertility
Ming-Huei LinSheng-Hsiang Li

Abstract

We report a secreted serine protease inhibitor Kazal-type-like (SPINKL) protein. The SPINKL protein was purified from mouse seminal vesicle secretions through a series of steps, including ion-exchange chromatography on a diethylaminoethyl-Sephacel column, gel filtration on a Sephadex G-75 column, and ion-exchange HPLC on a Q strong anion exchange column. Further analysis identified several SPINKL proteins with various N-linked carbohydrates. The SPINKL protein has six conserved cysteine residues that are nearly identical to those of members of the SPINK protein family. It was noted that the SPINKL protein showed no inhibitory activities against common serine proteases such as trypsin, chymotrypsin, subtilisin, or elastase. Spinkl mRNA and SPINKL proteins were found to be primarily expressed in seminal vesicles. Immunohistochemistry revealed that the SPINKL protein occurred in the luminal fluid and mucosal epithelium of the seminal vesicles and was regulated by testosterone. The SPINKL protein was able to bind onto sperm and enhance sperm motility. Also, it was able to suppress BSA-stimulated sperm capacitation and block sperm-oocyte interactions in vitro, suggesting that SPINKL may be a decapacitation factor.

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Citations

Sep 28, 2013·Molecular Human Reproduction·R John Aitken, Brett Nixon
Jan 10, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·L ZalazarA Cesari
Oct 31, 2012·Molecular Reproduction and Development·Brooke A Laflamme, Mariana F Wolfner
Oct 26, 2012·Journal of Cellular Biochemistry·Huan-Chin TsengSheng-Hsiang Li
Jun 15, 2013·The Prostate·Chad M McKeeRuth J Muschel
Aug 13, 2015·Experimental Biology and Medicine·Shuting ChengZhengrong Wang
Jan 10, 2019·Zygote : the Biology of Gametes and Early Embryos·Gabriela Hernández-Silva, Mayel Chirinos
Jan 31, 2018·Reproductive Biology and Endocrinology : RB&E·Robert Kuo-Kuang LeeSheng-Hsiang Li
Jun 13, 2020·Biology of Reproduction·Prashanth Anamthathmakula, Wipawee Winuthayanon
Mar 25, 2016·The Journal of Reproduction and Development·Yu-Wen KuoPei Shiue J Tsai
May 23, 2018·International Journal of Molecular Sciences·Sheng-Hsiang LiRobert Kuo-Kuang Lee
Apr 30, 2019·Molecular Reproduction and Development·Aurore ThélieElisabeth Blesbois
Jan 23, 2018·Experimental Animals·Yoshitaka FujiharaMasahito Ikawa
Jul 1, 2017·BMC Veterinary Research·Yu-Wen KuoPei-Shiue Jason Tsai
Jul 18, 2019·Reproductive Medicine and Biology·Taichi Noda, Masahito Ikawa
Sep 23, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Joanne WongSebastian Dworkin
Oct 27, 2020·Frontiers in Cell and Developmental Biology·Lucia ZalazarAndreina Cesari
Jan 21, 2021·Journal of Cellular Biochemistry·Chung-Mao OuSheng-Hsiang Li
Sep 16, 2021·Molecular Human Reproduction·Shiyam Sundar RamachandranSudhakar Gandhi Paramasivam
Oct 10, 2021·BMC Genomics·David A Skerrett-ByrneJohn E Schjenken

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