SPIO labeling of endothelial cells using ultrasound and targeted microbubbles at diagnostic pressures

PloS One
Ilya SkachkovKlazina Kooiman

Abstract

In vivo cell tracking of therapeutic, tumor, and endothelial cells is an emerging field and a promising technique for imaging cardiovascular disease and cancer development. Site-specific labeling of endothelial cells with the MRI contrast agent superparamagnetic iron oxide (SPIO) in the absence of toxic agents is challenging. Therefore, the aim of this in vitro study was to find optimal parameters for efficient and safe SPIO-labeling of endothelial cells using ultrasound-activated CD31-targeted microbubbles for future MRI tracking. Ultrasound at a frequency of 1 MHz (10,000 cycles, repetition rate of 20 Hz) was used for varying applied peak negative pressures (10-160 kPa, i.e. low mechanical index (MI) of 0.01-0.16), treatment durations (0-30 s), time of SPIO addition (-5 min- 15 min with respect to the start of the ultrasound), and incubation time after SPIO addition (5 min- 3 h). Iron specific Prussian Blue staining in combination with calcein-AM based cell viability assays were applied to define the most efficient and safe conditions for SPIO-labeling. Optimal SPIO labeling was observed when the ultrasound parameters were 40 kPa peak negative pressure (MI 0.04), applied for 30 s just before SPIO addition (0 min). Compared to...Continue Reading

References

Dec 1, 1969·Experimental Cell Research·P R Clarke, C R Hill
Jan 1, 1980·Ultrasound in Medicine & Biology·Y K FuG E Kaufman
Oct 1, 1994·Immunology Today·H M DeLisserS M Albelda
Oct 31, 1998·European Journal of Ultrasound : Official Journal of the European Federation of Societies for Ultrasound in Medicine and Biology·I HrazdiraM Dolníková
Apr 7, 2000·The American Journal of Pathology·H HashizumeD M McDonald
Feb 5, 2002·Journal of Clinical Pathology·A SternbergG Groisman
May 23, 2002·Echocardiography·Nico de JongPeter Frinking
Mar 28, 2003·Ultrasound in Medicine & Biology·Loreto B FerilShinobu Nakamura
Apr 12, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Peter J Newman, Debra K Newman
Apr 13, 2004·Investigative Radiology·Alexander L KlibanovGary H Brandenburger
Jun 7, 2005·Cell·Douglas R Green
Nov 2, 2005·The Journal of Pharmacy and Pharmacology·L Harivardhan Reddy
Jan 18, 2006·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Erik M ShapiroAlan P Koretsky
Apr 14, 2006·Neoplasia : an International Journal for Oncology Research·Barjor GimiZaver M Bhujwalla
Sep 5, 2006·Biophysical Journal·Claus-Dieter OhlDetlef Lohse
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Jul 27, 2007·Annual Review of Biomedical Engineering·Katherine FerraraMark Borden
Feb 2, 2008·IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control·P A DaytonK W Ferrara
Aug 30, 2008·Ultrasound in Medicine & Biology·Douglas L Miller, Chunyan Dou
Aug 30, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fang YangJunru Wu
Jun 12, 2009·Medical & Biological Engineering & Computing·Alexander L Klibanov
Aug 13, 2009·European Radiology·Monique R BernsenGabriel P Krestin
Aug 29, 2009·Clinical Hemorheology and Microcirculation·Christian Greis
Apr 15, 2010·Current Topics in Medicinal Chemistry·Hao HongWeibo Cai
Jun 4, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Anna YudinaChrit T W Moonen
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Nohyun LeeTaeghwan Hyeon
Apr 26, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Klazina KooimanNico de Jong
May 24, 2011·Ultrasound in Medicine & Biology·Hendrik J VosNico de Jong
Aug 6, 2011·Ultrasound in Medicine & Biology·Marlies OverveldeMichel Versluis
Oct 29, 2011·Ultrasound in Medicine & Biology·Wenjing ZhongAlfred C H Yu
Dec 22, 2011·Journal of Clinical Bioinformatics·Hong Chen, Xiangdong Wang
Jan 31, 2012·Imaging in Medicine·Yingli FuDara L Kraitchman
Feb 22, 2012·Ultrasound in Medicine & Biology·Christophoros Mannaris, Michalakis A Averkiou
May 19, 2012·JACC. Cardiovascular Imaging·Martin Rodriguez-PorcelRobert D Simari
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhenzhen FanCheri X Deng

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection

Software Mentioned

ImageJ
SonoVue

Related Concepts

Related Feeds

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.