Spironolactone in Combination with α-glycosyl Isoquercitrin Prevents Steatosis-related Early Hepatocarcinogenesis in Rats through the Observed NADPH Oxidase Modulation

Toxicologic Pathology
Hirotada MurayamaToshinori Yoshida

Abstract

Administration of the diuretic, spironolactone (SR), can inhibit chronic liver diseases. We determined the effects of SR alone or in combination with the antioxidant α-glycosyl isoquercitrin (AGIQ) on hyperlipidemia- and steatosis-related precancerous lesions in high-fat diet (HFD)-fed rats subjected to a two-stage hepatocarcinogenesis model. Rats were fed with control basal diet or HFD, which was administered with SR alone or in combination with an antioxidant AGIQ in drinking water. An HFD increased body weight, intra-abdominal fat (adipose) tissue weight, and plasma lipids, which were reduced by coadministration of SR and AGIQ. SR and AGIQ coadministration also reduced hepatic steatosis and preneoplastic glutathione S-transferase placental form-positive foci, in association with decrease in NADPH oxidase (NOX) subunit p22phox-positive cells and an increase in active-caspase-3-positive cells in the foci. Hepatic gene expression analysis revealed that the coadministration of SR and AGIQ altered mRNA levels of lipogenic enzymes ( Scd1 and Fasn), antioxidant-related enzymes ( Catalase), NOX component ( P67phox), and anti-inflammatory transcriptional factor ( Pparg). Our results indicated that SR in combination with AGIQ had the ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 26, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·David E KleinerUNKNOWN Nonalcoholic Steatohepatitis Clinical Research Network
Aug 30, 2006·Translational Research : the Journal of Laboratory and Clinical Medicine·Genro FujisawaShun Ishibashi
Jan 4, 2007·Hypertension·Shigeru ShibataToshiro Fujita
Dec 15, 2007·Endocrinology·Fumiko IwashimaYukio Hirata
Nov 13, 2008·International Journal of Cancer. Journal International Du Cancer·Yan WangXiang-Dong Wang
Mar 17, 2010·Progress in Cardiovascular Diseases·Adam Whaley-ConnellJames R Sowers
Jun 1, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jun YuJoseph J Y Sung
Dec 27, 2011·Journal of Acupuncture and Meridian Studies·M Suriyavathana Vedanarayanan, Nandhini Krishnan
Feb 22, 2012·Journal of the American Society of Hypertension : JASH·Tamara M ParaviciniRhian M Touyz
Aug 24, 2012·The Journal of Nutrition·Eder MarcolinMaría J Tuñón
Jul 3, 2013·Aging Cell·Fareeba SheedfarManlio Vinciguerra
Oct 17, 2013·American Journal of Physiology. Endocrinology and Metabolism·Tsutomu WadaToshiyasu Sasaoka
Dec 11, 2013·European Journal of Medical Research·Ryo KumamotoHirohito Tsubouchi
Apr 1, 2014·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Shintaro HaraMakoto Shibutani
Apr 1, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kateřina ValentováVladimír Křen
May 8, 2014·International Journal of Experimental Pathology·Sharon KingStewart Fleming
Dec 17, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Diptadip DattaroySaurabh Chatterjee
Oct 31, 2015·Experimental Cell Research·Rotonya M Carr, Rexford S Ahima
Feb 10, 2016·Digestive Diseases and Sciences·Samir SofticC Ronald Kahn
Oct 30, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Abraham NyskaRobert R Maronpot
Oct 30, 2016·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Toshinori YoshidaMakoto Shibutani
Feb 22, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wei WangBin Gao

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