Spiroquinazolinone-induced cytotoxicity and apoptosis in K562 human leukemia cells: alteration in expression levels of Bcl-2 and Bax

The Journal of Toxicological Sciences
Majid GhasemianMohammad Ali Hosseinpour Feizi

Abstract

Spiroquinazolinone compounds have been considered as a new series of potent apoptosis-inducing agents. In this study, anti-proliferative and apoptotic effects of the derivatives from the spiroquinazolinone family were investigated in the human chronic myeloid leukemia K562 cells. The K562 cells were treated with various concentrations of the spiroquinazolinone (10-300 µM) for 3 days and cell viability was determined by MTT growth inhibition assay. 4t-QTC was more active among these compounds with IC50 of 50 ± 3.6 µM and was selected for further studies. Apoptosis, as the mechanism of cell death was investigated morphologically by acridine orange/ethidium bromide (AO/EtBr) double staining, cell surface expression assay of phosphatidyl serine by Annexin V/PI technique, as well as the formation of DNA ladder. The K562 cells underwent apoptosis upon a single dose (at IC50 value) of the 4t-QTC compound, and over-expressed caspase-3 expression by more than 1.7-fold, following a 72 hr treatment. Furthermore, RT-PCR and Western blot analysis revealed that treatment of the K562 cells with 4t-QTC down-regulates and up-regulates the expression of Bcl-2 (anti-apoptotic) and Bax (pro-apoptotic), respectively. Based on the present data, it s...Continue Reading

References

Sep 12, 1998·Science·J M Adams, S Cory
Aug 31, 2000·Annual Review of Biochemistry·A StrasserV M Dixit
May 1, 2002·The Journal of Cell Biology·Girish V PutchaEugene M Johnson
Dec 4, 2003·Mutation Research·Marisa D Pulido, Alan R Parrish
Nov 5, 2005·Acta Biochimica Et Biophysica Sinica·Ting-Jun FanJin Liang
Jun 9, 2006·Cancer Biology & Therapy·Elena CubedoJesús Gacía-Foncillas
Jun 5, 2007·The International Journal of Biochemistry & Cell Biology·Mohammad Amin MoosaviAbbas Lotfi
Jul 17, 2007·Current Opinion in Immunology·Jerry M Adams, Suzanne Cory
Mar 19, 2008·Biochemical Pharmacology·Malgorzata CzyzMalgorzata Sztiller-Sikorska
Apr 28, 2009·Leukemia Research·Hong-Yun JiaWenlin Huang
Jul 31, 2009·Oncogene·L P BillenD W Andrews
Feb 16, 2010·Molecular Aspects of Medicine·Shinichi Sakamoto, Natasha Kyprianou
Apr 20, 2010·Biochemical and Biophysical Research Communications·Lin ShiYing Wang
Jul 6, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Aliny Pereira de LimaElisângela de Paula Silveira Lacerda
Jan 7, 2011·Journal of Nucleic Acids·Rajesh P RastogiRajeshwar P Sinha
Feb 23, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jessica PlatiRoya Khosravi-Far
Sep 24, 2011·Advances in Hematology·Flora TzifiAndreas Scorilas
Jun 12, 2012·Aging·Bartolo FavaloroVincenzo De Laurenzi

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Citations

Oct 8, 2016·Biologicals : Journal of the International Association of Biological Standardization·Sanam AramiNasser Samadi
May 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natarajan ArumugamJ Carlos Menéndez
Sep 8, 2019·Journal of Cell Communication and Signaling·Fatemeh AsgariNooshin Haghighipour
Oct 16, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Xiaofang WangChenguang Zhang
Nov 24, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Debanjana BiswalSwarnendu Chandra
Jul 29, 2015·World Journal of Gastroenterology : WJG·Hai-Bo ZhouZhi-Gang Chen

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