Spitzenkörper, vacuoles, ring-like structures, and mitochondria of Phanerochaete velutina hyphal tips visualized with carboxy-DFFDA, CMAC and DiOC6(3)

Mycological Research
Xueying ZhuangAnne E Ashford

Abstract

Growth and organelle morphology in the wood rotting basidiomycete fungus Phanerochaete velutina were examined in Petri dishes, on agar-coated slides, and in submerged cultures, using DIC, fluorescence and four-dimensional (4-D; x,y,z,t) confocal microscopy, with several fluorescent probes. Phanerochaete is ideal for this work because of its fast growth, robustness, and use in a wide range of other studies. The probe carboxy-DFFDA, widely used for labelling vacuoles, has no effect either on hyphal tip extension or colony growth at the concentrations usually applied in labelling experiments. Carboxy-DFFDA labels the vacuoles and these form a tubular reticulum in hyphal tip cells. The probe also labels extremely small vesicles (punctate fluorescence) in the apex of tip cells, the Spitzenkörper, and short tubules that undergo sequences of characteristic movements and transformations to produce various morphologies, including ring-like structures. Their location and behaviour suggest that they are a distinct group of structures, possibly a subset of vacuoles, but as yet to be fully identified. Regular incursions of tubules extending from these structures and from the vacuolar reticulum into the apical dome indicate the potential for...Continue Reading

References

Oct 1, 1978·Canadian Journal of Microbiology·L Hofherr
Feb 20, 1998·Fungal Genetics and Biology : FG & B·W G AllawayA R Hardham
Apr 4, 1998·Molecular Biology of the Cell·E C GaynorT R Graham
Sep 22, 1998·Fungal Genetics and Biology : FG & B·L ColeA E Ashford
Mar 17, 2001·Molecular Biology of the Cell·A KranzR Kölling
Sep 12, 2002·Fungal Genetics and Biology : FG & B·Mamoru OhnedaKatsuhiko Kitamoto
Aug 4, 2004·Fungal Genetics and Biology : FG & B·Meritxell Riquelme, Salomon Bartnicki-Garcia
Nov 3, 2004·Advances in Microbial Physiology·István PócsiMichel J Penninckx
Feb 11, 2005·Eukaryotic Cell·Steven D HarrisMichelle Momany
Apr 7, 2005·Archives of Microbiology·Cristina G Reynaga-Peña, Salomón Bartnicki-García
Aug 9, 2005·Applied and Environmental Microbiology·Mamoru OhnedaKatsuhiko Kitamoto
Nov 28, 2006·Fungal Genetics and Biology : FG & B·Cynthia L Araujo-PalomaresMeritxell Riquelme
Jun 8, 2007·The Journal of Biological Chemistry·Mari ValkonenRory R Duncan
Aug 28, 2007·Biochemical and Biophysical Research Communications·Akinori TatsumiKatsuhiko Kitamoto

❮ Previous
Next ❯

Citations

Sep 15, 2015·Fungal Genetics and Biology : FG & B·G Vidal-Diez de UlzurrunB De Baets
Apr 6, 2012·Plant Signaling & Behavior·Vasileios Fotopoulos
Oct 11, 2015·Eukaryotic Cell·Barry J BowmanEmma Jean Bowman
Apr 3, 2020·Mycologia·Igor MazheikaOlga Kamzolkina
Jun 23, 2012·Journal of Agricultural and Food Chemistry·Francisca AcevedoThierry Chardot

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.