Abstract
We developed and optimized a novel gene delivery vector constructed electrostatically with an anionic biological component and a cationic biological component. Cationic binary complexes of plasmid DNA (pDNA) with novo-protamine sulfate as a medical product (PRT complexes) demonstrated high gene expression with minimal cytotoxicity, likely related with its total cationic charge. Subsequently, anionic compounds were added to the PRT complexes to form ternary complexes with neutral or anionic charges. Among the anionic compounds examined, chondroitin sulfate sodium (CS) as a medical product encapsulated the PRT complexes to produce stable ternary complexes (CS complexes) at charge ratios of ≥4 with pDNA. CS complexes exhibited high gene expression without cytotoxicity in mouse melanoma cell line, B16-F10 cells, in vitro. An inhibition study with endocytosis inhibitors suggested that PRT complexes were mainly taken up by caveolae-mediated endocytosis, and CS complexes were mainly taken up by clathrin-mediated endocytosis in B16-F10 cells. We found that CS complexes including pDNA encoding Oplophorus gracilirostris luciferase induced selective gene expression in the spleen after intravenous administration into ddY male mice. Thus, w...Continue Reading
References
Feb 12, 1998·Gene Therapy·F L SorgiL Huang
Dec 20, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anas El-Aneed
Jan 1, 1950·The Journal of Experimental Medicine·M E KAPLANH W DEANE
Nov 17, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shubiao ZhangZongshi Li
Feb 22, 2005·Advanced Drug Delivery Reviews·Shiroh Futaki
Mar 2, 2006·Pharmacological Reviews·Ikramy A KhalilHideyoshi Harashima
Feb 24, 2009·Biomaterials·Tomoaki KurosakiHitoshi Sasaki
Oct 22, 2009·Biotechnology Journal·Atul PathakKailash Chand Gupta
Mar 17, 2010·International Journal of Pharmaceutics·Hong YuanFu-Qiang Hu
Sep 25, 2010·Biomaterials·Jing YaoXiaojing Sun
May 12, 2011·Molecular Pharmaceutics·Tomoaki KurosakiTakashi Kitahara
Sep 28, 2012·Journal of Applied Biomaterials & Functional Materials·Daniele PezzoliGabriele Candiani
Oct 2, 2012·Acta Biomaterialia·Wahid KhanAbraham J Domb
Feb 21, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Yoshikazu InohMamoru Nakanishi
Jul 19, 2013·Biomaterials·Huining HeVictor C Yang
Nov 6, 2013·Biological & Pharmaceutical Bulletin·Kosuke KandaHitoshi Sasaki
Nov 30, 2013·International Journal of Pharmaceutics·D IbraheemH Fessi
Jan 28, 2014·Archives of Disease in Childhood·David K ArmstrongEric W F W Alton
Mar 29, 2014·Molecular Pharmaceutics·Tomoaki KurosakiHitoshi Sasaki
May 13, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Yukinobu KodamaHitoshi Sasaki
Jun 2, 2015·Current Pharmaceutical Design·Hui Yi XueHo Lun Wong
Nov 20, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·José Vicente González-AramundizNoemi Stefánia Csaba
Citations
Sep 8, 2020·Angewandte Chemie·Christoph O FranckLjiljana Fruk
Jan 11, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Seigo KimuraHideyoshi Harashima
Mar 10, 2021·Drug Delivery·Yukinobu KodamaHitoshi Sasaki
Nov 28, 2021·Pharmaceutics·Tomoaki KurosakiYukinobu Kodama