Splice variants of the P2X7 receptor reveal differential agonist dependence and functional coupling with pannexin-1

Journal of Cell Science
Xing Jian XuRuth D Murrell-Lagnado

Abstract

P2X7 receptors function as ATP-gated cation channels but also interact with other proteins as part of a larger signalling complex to mediate a variety of downstream responses that are dependent upon the cell type in which they are expressed. Receptor-mediated membrane permeabilization to large molecules precedes the induction of cell death, but remains poorly understood. The mechanisms that underlie differential sensitivity to NAD are also unknown. By studying alternative variants of the mouse P2X7 receptor we show that sensitivity to NAD is mediated through the P2X7k variant, which has a much more restricted distribution than the P2X7a receptor, but is expressed in T lymphocytes. The altered N-terminus and TM1 of the P2X7k receptor enhances the stability of the active state of this variant compared with P2X7a, thereby increasing the efficacy of NAD-dependent ADP ribosylation as measured by ethidium uptake, a rise in intracellular Ca(2+) and the activation of inward currents. Co-expression of P2X7k and P2X7a receptors reduced NAD sensitivity. P2X7k-receptor-mediated ethidium uptake was also triggered by much lower BzATP concentrations and was insensitive to the P451L single nucleotide polymorphism. P2X7k-receptor-mediated ethid...Continue Reading

References

Jan 21, 2001·The Journal of Biological Chemistry·B J GuJ S Wiley
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L C DenlingerP J Bertics
Nov 15, 2001·The EMBO Journal·M KimA Surprenant
Feb 28, 2002·Molecular and Cellular Neurosciences·David BroughNancy J Rothwell
Jul 11, 2002·The Journal of Biological Chemistry·Heather L WilsonR Alan North
Aug 1, 2002·The Journal of General Physiology·Angela N EickhorstBaljit S Khakh
Sep 25, 2002·Physiological Reviews·R Alan North
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sahil AdriouchFriedrich Haag
Dec 24, 2002·The Journal of Biological Chemistry·Megan L SmartSteven Petrou
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip A VerhoefGeorge R Dubyak
Aug 12, 2004·FEBS Letters·Li BaoGerhard Dahl
Oct 8, 2004·American Journal of Physiology. Cell Physiology·R X FariaLuiz Anastácio Alves
May 18, 2005·Biochemical and Biophysical Research Communications·Boonlert CheewatrakoolpongScott Greenfeder
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Giulio CabriniFrancesco Di Virgilio
Jul 5, 2005·The Journal of Biological Chemistry·Amanda B MackenzieAnnmarie Surprenant
Aug 12, 2005·American Journal of Physiology. Cell Physiology·Lin-Hua JiangR Alan North
May 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Silviu LocoveiGerhard Dahl
Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Klaus OkkenhaugBart Vanhaesebroeck
Dec 19, 2006·Cellular Signalling·Samantha F Moore, Amanda B MacKenzie
Jan 24, 2007·FEBS Letters·Silviu LocoveiGerhard Dahl
Mar 8, 2007·Critical Reviews in Immunology·Lanfen Chen, Celia F Brosnan
Sep 6, 2007·Molecular Pharmacology·Chang GuoRuth D Murrell-Lagnado
Oct 12, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sahil AdriouchFriedrich Koch-Nolte
Oct 18, 2007·Journal of Cell Science·Omar S QureshiRuth D Murrell-Lagnado
Dec 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Simon R J TaylorJames I Elliott
Jul 4, 2008·American Journal of Physiology. Cell Physiology·R IglesiasE Scemes
Oct 15, 2008·The Journal of General Physiology·Zonghe YanStanko S Stojilkovic
Oct 15, 2008·Pflügers Archiv : European journal of physiology·Eliana ScemesPaolo Meda
Oct 24, 2008·American Journal of Physiology. Cell Physiology·Feng Qiu, Gerhard Dahl
Nov 22, 2008·The Journal of Pharmacology and Experimental Therapeutics·Weihong MaAnnmarie Surprenant
Feb 13, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Linda YipWolfgang G Junger
Feb 13, 2009·Purinergic Signalling·Pablo Pelegrin, Annmarie Surprenant
Feb 14, 2009·American Journal of Respiratory Cell and Molecular Biology·George A RansfordMatthias Salathe

❮ Previous
Next ❯

Citations

Sep 10, 2013·Molecular Neurobiology·Xian-Fang MengChun Zhang
Feb 28, 2014·TheScientificWorldJournal·Torben Madsen KvistNiklas Rye Jørgensen
Sep 17, 2014·Trends in Pharmacological Sciences·Beáta Sperlágh, Peter Illes
Feb 16, 2016·Nature Communications·Mausita KarmakarEric Pearlman
Mar 20, 2014·FEBS Letters·Samantha E Adamson, Norbert Leitinger
Aug 27, 2015·Immunobiology·Juan D Cortés-GarciaDiana P Portales-Pérez
Apr 14, 2016·The Journal of Cell Biology·Shyam SrivatsRuth D Murrell-Lagnado
Aug 2, 2015·Neuropharmacology·Chloé HabermacherThomas Grutter
Oct 11, 2014·Mediators of Inflammation·Pablo J SáezJuan C Sáez
May 23, 2015·Autonomic Neuroscience : Basic & Clinical·Amelia R HowarthMatthew A Bailey
Jun 5, 2015·Frontiers in Immunology·Björn RissiekSahil Adriouch
Jan 15, 2014·Channels·Yu-Hsin ChiuDouglas A Bayliss
Oct 9, 2012·Neuron·Baljit S Khakh, R Alan North
Apr 20, 2017·International Journal of Molecular Sciences·Filippo MolicaBrenda R Kwak
Aug 10, 2018·Multiple Sclerosis : Clinical and Laboratory Research·Heidi HögelLaura Airas
Oct 26, 2018·Annual Review of Physiology·Ralf Schmid, Richard J Evans
Oct 5, 2014·The Journal of Biological Chemistry·Lucy E RobinsonRuth D Murrell-Lagnado
Mar 6, 2019·Clinical and Experimental Pharmacology & Physiology·Camila Guerra Martinez
Jan 25, 2019·Annual Review of Immunology·Joel LindenGerhard Dahl
Aug 23, 2019·Physiological Reviews·Volker VallonBellamkonda K Kishore
Dec 22, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuta RyodenShigekazu Nagata
Jul 18, 2020·Medicinal Research Reviews·Francesco CalzaferriAntonio G García
Jun 15, 2014·Pharmacological Reviews·Rachael BartlettRonald Sluyter
Oct 2, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Brandon G ShokoplesErnesto L Schiffrin
Feb 4, 2015·The Journal of Biological Chemistry·Xin LiangTerrance M Egan
Jul 17, 2020·International Journal of Molecular Sciences·Elizabeth Rivas-YáñezDaniela Sauma
Aug 16, 2018·Frontiers in Molecular Neuroscience·Laurie PeveriniThomas Grutter
Jun 27, 2020·Frontiers in Immunology·Fabio Grassi
Aug 24, 2019·Frontiers in Molecular Neuroscience·Robin KoppAnnette Nicke
Nov 14, 2020·International Journal of Molecular Sciences·María Ángeles Martínez-CuestaÁngeles Álvarez
Jun 15, 2021·Frontiers in Cellular Neuroscience·Ágatha Oliveira-GiacomelliHenning Ulrich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved