PMID: 9450342Feb 5, 1998Paper

Splicing features in maize streak virus virion- and complementary-sense gene expression

The Plant Journal : for Cell and Molecular Biology
E A WrightMargaret I Boulton

Abstract

The single-stranded DNA geminiviruses produce transcripts from both strands (virion- and complementary-sense) of a nuclear double-stranded DNA molecule. In maize streak virus (MSV)-infected maize plants, approximately 80% of the complementary-sense transcripts produce the C1 protein, whilst the remaining 20% are spliced to remove a 92 nt intron and produce a C1:C2 fusion protein (Rep). Disruption of the complementary-sense 3' splice site abolished virus replication. The majority of the virion-sense transcripts initiated one nucleotide upstream of the V1 (movement protein) gene and a minority a further 141 nucleotides upstream. A 76 nt intron, with features typical of plant introns, was identified within the V1 gene, upstream of the coat protein gene. Spliced and unspliced forms of each virion-sense transcript were produced, but they differed in splicing efficiency. Approximately 50% of the major transcript and less than 10% of the minor transcript were processed. Mutagenesis of the consensus 5' splice site in the V1 gene resulted in the use of alternative cryptic splice sites, confirming the importance of splicing for MSV infection. Spliced virion-sense transcripts were also identified in tissue infected with the closely-relate...Continue Reading

Citations

Jun 21, 2008·Archives of Virology·N NahidR W Briddon
Jan 4, 2001·Virus Research·N A Bosque-Pérez
Apr 25, 2000·Plant Science : an International Journal of Experimental Plant Biology·G MazithulelaM I Boulton
Oct 9, 2013·Nature Reviews. Microbiology·Linda Hanley-BowdoinShahid Mansoor
Jun 27, 2012·Journal of Virology·Björn KrenzKeith L Perry
Dec 28, 2012·BMC Evolutionary Biology·Adérito L MonjaneDarren P Martin
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·William Gordon-KammBrian A Larkins
Oct 12, 1999·Molecular Plant-microbe Interactions : MPMI·H LiuJ W Davies
May 3, 2001·Molecular Plant-microbe Interactions : MPMI·K NikovicsG V Horváth
Feb 14, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Maketalena F MaleArvind Varsani
Apr 3, 2012·Virus Research·Anisha DayaramArvind Varsani
Jul 1, 2007·Molecular Plant Pathology·Javaria QaziRob W Briddon
Apr 26, 2013·Molecular Plant Pathology·Vincent N Fondong
Mar 5, 2015·Phytopathology·Mysore R SudarshanaMarc F Fuchs
Jul 31, 2012·Virus Research·Simona KrabergerArvind Varsani
Sep 3, 2013·Virus Research·Alyssa SikorskiArvind Varsani
Jan 15, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Simona KrabergerArvind Varsani
Feb 24, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Anisha DayaramArvind Varsani
May 18, 2016·Virology·B K BorahI Dasgupta
May 24, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Olivia SteelArvind Varsani
Apr 19, 2018·The Plant Journal : for Cell and Molecular Biology·Tal Dahan-MeirAvraham A Levy
Dec 20, 2002·Biotechnology and Bioengineering·Tsafrir S MorHugh S Mason
Feb 19, 2019·The Journal of General Virology·José Vargas-AsencioJeremy R Thompson
Aug 11, 2004·The Journal of General Virology·Rémy FroissartStéphane Blanc
Oct 2, 2017·BMC Bioinformatics·José Cleydson F SilvaFabio R Cerqueira
Sep 3, 2020·MBio·Ignacio de la HigueraKenneth M Stedman
Feb 19, 2017·Archives of Virology·Arvind VarsaniDarren P Martin

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