Mar 29, 2008

Splicing regulation: from a parts list of regulatory elements to an integrated splicing code

RNA
Zefeng Wang, Christopher B Burge

Abstract

Alternative splicing of pre-mRNAs is a major contributor to both proteomic diversity and control of gene expression levels. Splicing is tightly regulated in different tissues and developmental stages, and its disruption can lead to a wide range of human diseases. An important long-term goal in the splicing field is to determine a set of rules or "code" for splicing that will enable prediction of the splicing pattern of any primary transcript from its sequence. Outside of the core splice site motifs, the bulk of the information required for splicing is thought to be contained in exonic and intronic cis-regulatory elements that function by recruitment of sequence-specific RNA-binding protein factors that either activate or repress the use of adjacent splice sites. Here, we summarize the current state of knowledge of splicing cis-regulatory elements and their context-dependent effects on splicing, emphasizing recent global/genome-wide studies and open questions.

  • References133
  • Citations382

References

Mentioned in this Paper

Nested Transcripts
Primary RNA Transcript
Gene Expression
Genes, Regulator
Proteomics
Nuclear mRNA Cis Splicing, via Spliceosome
RNA Splicing
Silencer Elements, Transcriptional
Introns
RBMS3 gene

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