Splicing-related catalysis by protein-free snRNAs

Nature
Saba Valadkhan, James L Manley

Abstract

Removal of intervening sequences from eukaryotic messenger RNA precursors is carried out by the spliceosome, a complex assembly of five small nuclear RNAs (snRNAs) and a large number of proteins. Although it has been suggested that the spliceosome might be an RNA enzyme, direct evidence for this has been lacking, and the identity of the catalytic domain of the spliceosome is unknown. Here we show that a protein-free complex of two snRNAs, U2 and U6, can bind and position a small RNA containing the sequence of the intron branch site, and activate the branch adenosine to attack a catalytically critical domain of U6 in a reaction that is related to the first step of splicing. Our data provide direct evidence for the catalytic potential of spliceosomal snRNAs.

References

Jul 25, 1992·Nucleic Acids Research·P Fabrizio, J Abelson
Dec 1, 1990·Genes & Development·H D MadhaniC Guthrie
Nov 29, 1974·Science·I R Lehman
Apr 1, 1995·Genes & Development·J S Sun, J L Manley
Nov 1, 1996·Nucleic Acids Research·Z Huang, J W Szostak
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S W Santoro, G F Joyce
Apr 1, 1997·Trends in Biochemical Sciences·W Y Tarn, J A Steitz
Jul 1, 1997·Current Biology : CB·A Newman
Aug 21, 1997·Nature·E J SontheimerJ A Piccirilli
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·C WuH M Wang
Oct 4, 2000·Nature Structural Biology·C A Collins, C Guthrie

❮ Previous
Next ❯

Citations

Jul 17, 2008·Comparative and Functional Genomics·M E FaheyD G Higgins
Feb 13, 2003·Biochemistry·Dipali G SashitalSamuel E Butcher
Apr 5, 2012·Biochemistry·Clarisse van der FeltzDaniel A Pomeranz Krummel
Mar 12, 2013·Journal of the American Chemical Society·Teresa CarlomagnoBernd Simon
Jul 2, 2009·Journal of the American Chemical Society·Alethia A HostetterVictoria J DeRose
Jul 12, 2002·Nature·Jennifer A Doudna, Thomas R Cech
Nov 3, 2009·Nature Structural & Molecular Biology·Zhuojun GuoDavid Rueda
Nov 16, 2004·Nature Structural & Molecular Biology·Dipali G SashitalSamuel E Butcher
Mar 22, 2006·Chemical Communications : Chem Comm·Youngha RyuA Ian Scott
Apr 28, 2009·Molecular BioSystems·Chaim Wachtel, James L Manley
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Saba ValadkhanSarah Geisler
Jul 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Samuel E Butcher
Aug 29, 2002·The EMBO Journal·Shawna L HileyRichard A Collins
Mar 28, 2002·Nucleic Acids Research·Vivek AnantharamanL Aravind
Dec 20, 2002·Nucleic Acids Research·Raymond T O'Keefe
Oct 25, 2003·Nucleic Acids Research·Sandra Carriero, Masad J Damha
Mar 24, 2009·Nucleic Acids Research·Keith T GagnonStefan Franzen
Oct 13, 2011·Nucleic Acids Research·Ascensión Ariza-MateosJordi Gómez
Sep 21, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Joseph A Piccirilli, Yelena Koldobskaya
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Cindy L Will, Reinhard Lührmann
Apr 4, 2007·Genes & Development·Angela K HillikerJonathan P Staley
Apr 9, 2011·Science·Aniela WochnerPhilipp Holliger
Jun 11, 2004·Annual Review of Biochemistry·Gerald F Joyce
Mar 21, 2009·Annual Review of Biochemistry·Adam Roth, Ronald R Breaker
Nov 11, 2006·Annual Review of Genetics·Francisco Rodríguez-TrellesFrancisco J Ayala
Dec 18, 2013·Genome Biology·W Ford Doolittle
Feb 14, 2012·RNA·Jordan E BurkeSamuel E Butcher
Nov 26, 2008·RNA·Duncan J Smith, Maria M Konarska
Nov 26, 2008·RNA·Duncan J Smith, Maria M Konarska
Nov 26, 2008·RNA·Saba Valadkhan, James L Manley
Jun 18, 2003·RNA·Saba Valadkhan, James L Manley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.