Split Green Fluorescent Proteins: Scope, Limitations, and Outlook

Annual Review of Biophysics
Matthew G Romei, Steven G Boxer

Abstract

Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein-protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies.

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Methods Mentioned

BETA
protein folding
PCA
electron microscopy
biosensor
Fluorescence
GTPases
super-resolution microscopy
size exclusion chromato-graphy
X-ray

Software Mentioned

GRASP
BiFC

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