Oct 25, 2018

Split Selectable Markers

BioRxiv : the Preprint Server for Biology
Nathaniel JilletteAlbert W Cheng

Abstract

Selectable markers are widely adopted in transgenesis and genome editing for selecting engineered cells with desired genotype but are limited in choices. We present here split selectable markers each allowing for selection of multiple unlinked transgenes in the context of lentivirus-mediated transgenesis as well as CRISPR/Cas-mediated biallelic knock-ins. Future development of split selectable markers may support enrichment or selection of hyper-engineered cells containing tens of transgenes or genetic modifications.

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Mentioned in this Paper

Biological Markers
CRISPR-Cas Systems
Gene Editing
Fluorescent stain
Toxic Epidermal Necrolysis
Transgenes
Insula of Reil
Genotype Determination
Genetic Engineering
Antibiotics

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