PMID: 9542951May 23, 1998Paper

Spoligotyping followed by double-repetitive-element PCR as rapid alternative to IS6110 fingerprinting for epidemiological studies of tuberculosis

Journal of Clinical Microbiology
C SolaN Rastogi

Abstract

A total of 129 clinical isolates of Mycobacterium tuberculosis representing 91 patients were typed by a combination of direct-repeat (DR)-based spoligotyping and an inter-IS6110-PGRS (polymorphic GC-rich region)-PCR, also designated double-repetitive-element PCR (DRE-PCR). During the first phase of this investigation, 72 clinical strains representing 52 patients were initially typed by IS6110-restriction fragment length polymorphism (RFLP) and DR-RFLP, followed by spoligotyping and DRE-PCR. In the second phase of this investigation, the discriminating ability of spoligotyping plus DRE-PCR was studied for 57 isolates from 39 patients who were suspected to be epidemiologically linked, and the typing results were later confirmed by IS6110-RFLP and DR-RFLP analyses. The molecular clustering of the isolates remained identical irrespective of the methods used. These results show that the association of two PCR-based fingerprinting techniques for molecular epidemiology of tuberculosis has a discriminating ability similar to the IS6110-RFLP reference method.

References

May 1, 1995·Journal of Clinical Microbiology·C R FriedmanL W Riley
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Citations

Sep 24, 2013·BMC Infectious Diseases·Samantha RosasAmador Goodridge
Aug 17, 2005·Emerging Infectious Diseases·Andrea GoriFabio Franzetti
Oct 16, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H-L HsuP-R Hsueh
Jul 10, 2004·Journal of Clinical Microbiology·Karine BrudeyNalin Rastogi
Jun 18, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christophe SolaNalin Rastogi
May 29, 2001·Journal of Clinical Microbiology·A BanderaF Franzetti
Mar 29, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jitendra Prasad MathuriaShampa Anupurba
Nov 26, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P MoströmN Rastogi
Aug 12, 2009·Tuberculosis·Ana Bárbara Scholante SilvaPedro Eduardo Almeida da Silva

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