Spontaneous and induced aneuploidy in peripheral blood lymphocytes of patients with Alzheimer's disease

Human Genetics
Lucia MiglioreU Bonuccelli

Abstract

This study was aimed at assessing whether peripheral blood lymphocytes of patients with Alzheimer's disease (AD) show significant levels of aneuploidy and high percentages of cytogenetic events in vitro, indicating a predisposition to aneuploidy spontaneously, or after chemical treatment in vitro. A group of affected individuals and a group of unaffected, age-, sex- and smoking-habit-matched controls were identified. Lymphocytes were cultured for analysis of the following cytogenetic parameters: premature centromere division (PCD), satellite associations of acrocentric chromosomes (SA) and micronuclei (MN). In a subset of subjects, the fluorescence in situ hybridization (FISH) technique was combined with the MN assay, by means of a pancentromeric DNA probe for the detection of the presence of centric material. To evaluate the sensitivity to aneuploidogenic agents, in vitro treatment of lymphocytes of affected individuals was performed by adding griseofulvin, a chemical whose supposed target is microtubule-associated protein(s). Both the spontaneous frequency of MN and the frequency of PCD was significantly higher in patient cells than in controls. Furthermore, after application of the FISH technique, we found that the majority ...Continue Reading

Citations

Apr 25, 2012·Molecular Neurobiology·Thomas Arendt
May 8, 2004·Experimental Gerontology·Biljana Spremo-PotparevicVladan Bajic
Sep 2, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Lada ZivkovićVladan Bajić
Dec 18, 2010·Mutagenesis·Lucia MigliorePhilip Thomas
Apr 14, 2010·Health Physics·Dubravka JovicićNenad Bukvić
May 31, 2011·Journal of Biomedical Science·Jasmine P DevadhasanJeongho An
Mar 8, 2013·Seminars in Cell & Developmental Biology·Diane M Bushman, Jerold Chun
Nov 29, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A Abou-Eisha
Apr 5, 2011·Cellular & Molecular Biology Letters·Vladan P BajićXiongwei Zhu
Sep 29, 2012·Food Chemistry·Karunrat SakulnarmratIzabela Konczak
May 25, 2015·Neuroscience and Biobehavioral Reviews·Vladan BajicThomas Arendt
Apr 8, 2017·Journal of Alzheimer's Disease : JAD·Angeles GarciaSabine Mai
Feb 1, 2017·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Nan Young ChoKyoung Kon Kim
Aug 22, 2000·American Journal of Medical Genetics·M B PetersenM Mikkelsen
Mar 13, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Maxime FrançoisMichael Fenech
Feb 28, 2019·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Osvaldo RiveraMatthew E Talbert
Aug 20, 2005·Gerontology·Hamiyet Dönmez-AltuntaşYusuf Ozkul
Mar 31, 2015·Environmental and Molecular Mutagenesis·Ann ChuaMichael Fenech
Sep 15, 2020·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Daniela CamporezFlavia de Paula
Dec 4, 2019·Frontiers in Genetics·Huntington PotterAntoneta Granic
Jan 30, 2016·Disease Models & Mechanisms·Anne-Laure Bougé, Marie-Laure Parmentier
Dec 19, 2020·Scientific Reports·Hauke ReimannHenning Hintzsche
Aug 11, 2015·Mutation Research·Mirta MilicStefano Bonassi

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