Spontaneous and ligand-induced trafficking of CXC-chemokine receptor 4.

The Journal of Biological Chemistry
N I TarasovaC J Michejda

Abstract

A chimeric protein consisting of CXC-chemokine receptor 4 (CXCR4) and the green fluorescent protein (GFP) was used for studying receptor localization and trafficking in real time in stably transduced HeLa, U-937, CEM, and NIH/3T3 cells. CXCR4-GFP was fully active as a co-receptor in mediating human immunodeficiency virus (HIV) entry. Both CXCR4 and CXCR4-GFP were found to undergo significant spontaneous endocytosis. Only 51.5 +/- 7.8% of receptor molecules were found on the plasma membrane in CD4-positive cells, 43.9 +/- 8.5% were found in CD4-negative HeLa cells, 75.6 +/- 9.7% were found in U-937 cells, 72.5 +/- 7.9 were found in CEM cells, and almost none were found in in NIH/3T3 cells. Stromal cell-derived factor-1alpha induced rapid endocytosis of cell surface receptor molecules. A significant part of CXCR4 was targeted to lysosomes upon binding of the ligands, and recycling of internalized CXCR4 was not efficient. Only about 30% of receptor molecules recycled back to the cell surface in HeLa cells, 5% recycled in U937, and 10% recycled in CEM cells, suggesting that the protective effect of chemokines against HIV infection can be attributed not only to competition for binding but also to depletion of the co-receptor molecul...Continue Reading

References

Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·R AshworthP M Hinkle
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·C C BleulC R Mackay
Sep 1, 1996·The Journal of Experimental Medicine·C C BleulT A Springer
Feb 15, 1997·The Biochemical Journal·S K BöhmN W Bunnett
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z LuR W Doms
Jan 7, 1998·Frontiers in Bioscience : a Journal and Virtual Library·P D Bieniasz, B R Cullen
Apr 4, 1998·BioTechniques·R H StauberG N Pavlakis

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Citations

Sep 6, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Fabrizio VianelloMark C Poznansky
Jul 8, 2010·International Journal of Colorectal Disease·Shang-Chiung WangShih-Ching Chang
Dec 14, 2005·Breast Cancer Research and Treatment·Ombretta SalvucciMark Basik
Apr 25, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Italo MocchettiValeriya Avdoshina
Jul 17, 2003·Journal of Neuroimmunology·Amos BodnerRichard J Miller
Apr 29, 2000·Trends in Pharmacological Sciences·L Kallal, J L Benovic
Apr 17, 2003·Cytokine·Hema VaidyanathanSteven D Schwartzbach
Nov 21, 2000·The International Journal of Biochemistry & Cell Biology·A A Maghazachi
Mar 22, 2012·Journal of Proteome Research·Louic S VermeerA James Mason
Feb 14, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Arnt-Ove Hovden, Maja A Sommerfelt
Mar 30, 2001·The Journal of Biological Chemistry·G H FanA Richmond
Dec 10, 2008·The Journal of Biological Chemistry·Zinaida DubeykovskayaTimothy C Wang
Jan 31, 2002·AIDS·A Brelot, M Alizon
Jan 5, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·M B Huang, V C Bond
Jul 10, 1999·Immunological Reviews·A Pelchen-MatthewsM Marsh
Jun 8, 1999·Annual Review of Immunology·E A BergerJ M Farber
Feb 13, 2008·Virology Journal·Rajeshwari R Valiathan, Marilyn D Resh
Feb 26, 2010·Journal of Experimental & Clinical Cancer Research : CR·Yixin LiuBaocun Sun
Feb 18, 2010·PloS One·Ulrike NaumannMarcus Thelen
May 11, 2012·Future Medicinal Chemistry·Ryo MasudaNobutaka Fujii
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·G CzerwinskiC J Michejda
Feb 2, 2012·International Journal of Breast Cancer·David Hiller, Quyen D Chu
Apr 25, 2009·Leukemia & Lymphoma·Seong-Woo KimDeog-Yeon Jo
Aug 12, 2009·FEBS Letters·Verena SchwartzJürgen Bernhagen

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