Spontaneous and Vaccine-Induced Clearance of Mus Musculus Papillomavirus 1 Infection

Journal of Virology
Rosie JiangRichard B S Roden

Abstract

Mus musculus papillomavirus 1 (MmuPV1/MusPV1) induces persistent papillomas in immunodeficient mice but not in common laboratory strains. To facilitate the study of immune control, we sought an outbred and immunocompetent laboratory mouse strain in which persistent papillomas could be established. We found that challenge of SKH1 mice (Crl:SKH1-Hrhr) with MmuPV1 by scarification on their tail resulted in three clinical outcomes: (i) persistent (>2-month) papillomas (∼20%); (ii) transient papillomas that spontaneously regress, typically within 2 months (∼15%); and (iii) no visible papillomas and viral clearance (∼65%). SKH1 mice with persistent papillomas were treated by using a candidate preventive/therapeutic naked-DNA vaccine that expresses human calreticulin (hCRT) fused in frame to MmuPV1 E6 (mE6) and mE7 early proteins and residues 11 to 200 of the late protein L2 (hCRTmE6/mE7/mL2). Three intramuscular DNA vaccinations were delivered biweekly via in vivo electroporation, and both humoral and CD8 T cell responses were mapped and measured. Previously persistent papillomas disappeared within 2 months after the final vaccination. Coincident virologic clearance was confirmed by in situ hybridization and a failure of disease to r...Continue Reading

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Citations

Sep 5, 2017·Viruses·Jiafen HuNeil D Christensen
Dec 1, 2017·Viruses·Aayushi Uberoi, Paul F Lambert
Sep 26, 2017·The Journal of General Virology·Nancy M CladelJiafen Hu
Feb 14, 2020·Journal of Virology·Megan E Spurgeon, Paul F Lambert
Oct 18, 2019·ELife·Megan E Spurgeon, Paul F Lambert
May 18, 2018·Frontiers in Microbiology·Daniel HascheFrank Rösl
Dec 13, 2019·Virology·Alexandra D TorresPaul F Lambert
Jan 22, 2021·Journal of Virology·Frank StubenrauchRichard B S Roden

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Methods Mentioned

BETA
flow cytometry
enzyme-linked immunosorbent assay
ELISA
transfection
bioluminescence imaging
PCR
ELISAs

Software Mentioned

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