Spontaneous calcium waves in the developing enteric nervous system

Developmental Biology
Marlene M HaoHeather M Young

Abstract

The enteric nervous system (ENS) is an extensive network of neurons in the gut wall that arises from neural crest-derived cells. Like other populations of neural crest cells, it is known that enteric neural crest-derived cells (ENCCs) influence the behaviour of each other and therefore must communicate. However, little is known about how ENCCs communicate with each other. In this study, we used Ca(2+) imaging to examine communication between ENCCs in the embryonic gut, using mice where ENCCs express a genetically-encoded calcium indicator. Spontaneous propagating calcium waves were observed between neighbouring ENCCs, through both neuronal and non-neuronal ENCCs. Pharmacological experiments showed wave propagation was not mediated by gap junctions, but by purinergic signalling via P2 receptors. The expression of several P2X and P2Y receptors was confirmed using RT-PCR. Furthermore, inhibition of P2 receptors altered the morphology of the ENCC network, without affecting neuronal differentiation or ENCC proliferation. It is well established that purines participate in synaptic transmission in the mature ENS. Our results describe, for the first time, purinergic signalling between ENCCs during pre-natal development, which plays rol...Continue Reading

Citations

Mar 30, 2019·The Journal of Physiology·Nicolas R ChevalierOrestis Faklaris
May 16, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jesse J DiCelloDaniel P Poole
Dec 13, 2019·Nature Communications·Nikolai RakhilinXiling Shen
Sep 27, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Nicolas R Chevalier
Sep 10, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jaime P P FoongJoel C Bornstein

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