PMID: 375020Mar 9, 1979Paper

Spontaneous degradation of pRD1 DNA into unique size classes is recA dependent

Molecular & General Genetics : MGG
A PühlerW Wohlleben

Abstract

The his and nif genes of the P1 type plasmid pRD1 were lost at a high frequency in a recA+ but not in a recA- Escherichia coli host during growth in a non-selective medium. 92% of the His- Nif- segregants after 6 subcultures retained the genetic markers of the precursor plasmid RP4, while the remainder lost all of the pRD1 markers with the concomitant loss of ccc-DNA. Plasmids purified from the His- Nif- segregants resembled RP4 in the physical and genetic properties examined. The contour length of pRD1 DNA molecules from a recA- strain was 49 micrometer corresponding to a molecular weight of 101 Mdals and the buoyant density was 1.715 g/cm3. In contrast, the contour lengths of plasmid molecules isolated from a recA+ host carrying pRD1 fell into 3 size classes of 49, 19 and 2 micrometer corresponding to molecular weights of 101, 39 and 4 Mdals respectively and two DNA species of buoyant density 1.715 and 1.719 g/cm3 were observed.

References

Aug 21, 1975·Biochimica Et Biophysica Acta·U BernardH Küntzel
Feb 1, 1977·Journal of General Microbiology·J R Postgate, V Krishnapillai
Jan 1, 1974·Journal of General Microbiology·F C CannonS B Primrose
May 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R RadloffJ Vinograd
Jul 1, 1950·Journal of Bacteriology·B D DAVIS, E S MINGIOLI

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Citations

Jan 1, 1983·Molecular & General Genetics : MGG·W KlingmüllerM Singh
Apr 1, 1984·Archives of Microbiology·N A KozyrovskayaV A Kordyum
Jan 1, 1980·Molecular & General Genetics : MGG·E R Appelbaum, R A Kramer

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