Spontaneous Formation of Extensive Vessel-Like Structures in Murine Engineered Heart Tissue

Tissue Engineering. Part a
Andrea StoehrThomas Eschenhagen

Abstract

Engineered heart tissue (EHT) from primary heart cells contains endothelial cells (ECs), but the extent to which ECs organize into vessel-like structures or even functional vessels remains unknown and is difficult to study by conventional methods. In this study, we generated fibrin-based mini-EHTs from a transgenic mouse line (Cdh5-CreERT2 × Rosa26-LacZ), in which ECs were specifically and inducibly labeled by applying tamoxifen (EC(iLacZ)). EHTs were generated from an unpurified cell mix of newborn mouse hearts and were cultured under standard serum-containing conditions. Cre expression in 15-day-old EHTs was induced by addition of o-hydroxytamoxifen to the culture medium for 48 h, and ECs were visualized by X-gal staining. EC(iLacZ) EHTs showed a dense X-gal-positive vessel-like network with distinct tubular structures. Immunofluorescence revealed that ECs were mainly associated with cardiomyocytes within the EHT. EC(iLacZ) EHT developed spontaneous and regular contractility with forces up to 0.1 mN. Coherent contractility and the presence of an extensive vessel-like network were both dependent on the presence of animal sera in the culture medium. Contractile EC(iLacZ) EHTs successfully served as grafts in implantation studie...Continue Reading

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Citations

Jun 16, 2017·Lab on a Chip·Boyang Zhang, Milica Radisic
Feb 14, 2017·Stem Cells International·Tilman ZieglerChristian Kupatt
Apr 30, 2017·Circulation Research·Florian WeinbergerThomas Eschenhagen
Nov 6, 2018·Nanotechnology·Dawn S Y LinBoyang Zhang
Dec 21, 2018·Expert Opinion on Biological Therapy·Tilman U EsserFelix B Engel
Jun 22, 2019·Journal of Muscle Research and Cell Motility·Thomas J Owen, Sian E Harding
May 10, 2019·APL Bioengineering·Richard J Mills, James E Hudson
Oct 2, 2019·Bioengineering & Translational Medicine·Lauren A HerronHasan E Abaci
Feb 23, 2021·Frontiers in Pharmacology·Moustafa H MekiTamer M A Mohamed
Sep 23, 2020·Trends in Biotechnology·Karl T WagnerMilica Radisic

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Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

CTMV

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