Spontaneous generation and survival of blood dendritic cells in mononuclear cell culture without exogenous cytokines.

Blood
Christopher S K HoJ Alejandro López

Abstract

Studies on purified blood dendritic cells (DCs) are hampered by poor viability in tissue culture. We, therefore, attempted to study some of the interactions/relationships between DCs and other blood cells by culturing unseparated peripheral blood mononuclear cell (PBMC) preparations in vitro. Flow cytometric techniques were used to undertake a phenotypic and functional analysis of DCs within the cultured PBMC population. We discovered that both the CD11c(+) and CD11c(-) CD123(hi) DC subsets maintained their viability throughout the 3-day culture period, without the addition of exogenous cytokines. This viability was accompanied by progressive up-regulation of the surface costimulatory (CD40, CD80, CD86) and activation (CMRF-44, CMRF-56, CD83) molecules. The survival and apparent production of DCs in PBMC culture (without exogenous cytokines) and that of sorted DCs (with cytokines) were evaluated and compared by using TruCOUNT analysis. Absolute DC counts increased (for CD123(hi) and CD11c(+) subsets) after overnight culture of PBMCs. Single-cell lineage depletion experiments demonstrated the rapid and spontaneous emergence of "new" in vitro generated DCs from CD14(+)/CD16(+) PBMC radioresistant precursors, additional to the pre...Continue Reading

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Citations

Mar 1, 2003·Journal of Immunological Methods·J Alejandro LópezDerek N J Hart
Feb 7, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Vito R CicinnatiSusanne Beckebaum
Jul 1, 2011·Blood·Emanuela MartinuzziRoberto Mallone
Jan 19, 2006·Breast Cancer Research : BCR·Alberto Pinzon-CharryJ Alejandro López
Mar 10, 2006·Breast Cancer Research : BCR·Alberto Pinzon-CharryJosé Alejandro López
May 9, 2012·World Journal of Gastroenterology : WJG·Kathleen F BensonGitte S Jensen
May 10, 2015·Biogerontology·Birgit WeinbergerBeatrix Grubeck-Loebenstein
Aug 15, 2006·Veterinary Immunology and Immunopathology·Viskam WijewardanaToshio Inaba
Jun 19, 2004·Cytotherapy·A M RiceD N J Hart
Jun 19, 2004·Cytotherapy·T H DoI M Svane
Oct 10, 2007·British Journal of Cancer·A Pinzon-CharryJ A López
Feb 2, 2011·International Archives of Allergy and Immunology·Anna-Karin EkmanLars-Olaf Cardell
Dec 1, 2004·Journal of Leukocyte Biology·Slavica VuckovicDerek N J Hart
Aug 21, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ingo FrickeSven Brandau
Apr 12, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marc DauerAndreas Eigler
May 22, 2020·JCI Insight·DiyaaElDin AshourManfred B Lutz
Nov 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Susanne BeckebaumVito R Cicinnati
May 7, 2021·Immunotherapy·Ming WuYu-Hua Li

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