PMID: 7334070Jan 1, 1981Paper

Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. IX. The quantitation of natural killer cell activity

Journal of Clinical Immunology
H F ProssM S Patterson

Abstract

On analysis of in vitro assays of human natural killer (NK) cell function the inadequacy of commonly used methods of expressing lytic activity was apparent. A comparison was made of the data obtained using modifications of two equations-the simple exponential fit and the von Krogh equations. Both of these equations were found to satisfy the following essential criteria for use in these assays. First, the majority of the results obtained in the chromium-release assay could be used in data reduction; second, the resultant "dose-response" curve was reduced to linearity; and third, a single numerical expression was obtained which was directly proportional to the cytotoxic activity. Of the two methods the more conventional exponential fit was found to be the simpler to use. The closeness of fit of the experimentally derived data to the ideal curves did not support the possibility that normal lymphocyte preparations contain suppressor cells capable of inhibiting NK activity. Data have also been presented showing that NK-sensitive targets could be categorized with respect to their susceptibility by comparing the slopes of the target cell survival curves obtained using the exponential fit equation. These observations are relevant to th...Continue Reading

References

Aug 15, 1976·International Journal of Cancer. Journal International Du Cancer·R K OldhamR B Herberman
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E Svedmyr, M Jondal
Mar 15, 1978·International Journal of Cancer. Journal International Du Cancer·H F ProssM G Baines
Jan 1, 1978·Oncology·M Menon, S S Stefani
Jul 1, 1979·British Journal of Cancer·P HerseyW H McCarthy
Jan 1, 1979·Immunological Reviews·R B HerbermanP Puccetti
Apr 15, 1975·International Journal of Cancer. Journal International Du Cancer·M Jondal, H Pross
Nov 15, 1976·International Journal of Cancer. Journal International Du Cancer·H F Pross, M G Baines
Dec 1, 1974·Journal of Immunological Methods·M DunkleyK Shortman
Feb 1, 1973·Journal of the National Cancer Institute·C B Lozzio, B B Lozzio
Oct 15, 1974·International Journal of Cancer. Journal International Du Cancer·J E de VriesP Rumke
Apr 1, 1980·The Journal of Experimental Medicine·J Y DjeuR B Herberman
Jan 1, 1980·Annals of the New York Academy of Sciences·R B HerbermanS Pestka
Jan 15, 1980·International Journal of Cancer. Journal International Du Cancer·P PuccettiR B Herberman

❮ Previous
Next ❯

Citations

Feb 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A FrancavillaT E Starzl
Jan 1, 1990·Journal of Clinical Laboratory Analysis·T L WhitesideR B Herberman
Jan 1, 1990·Journal of Clinical Laboratory Analysis·R E PollockE Lotzová
Jan 1, 1992·Journal of Clinical Laboratory Analysis·L H AndersonL W Klassen
Jul 31, 1998·Clinical Immunology and Immunopathology·P H LevineR B Herberman
Mar 1, 1993·Clinical & Experimental Metastasis·G G MeadowsL J Pfister
Oct 1, 1987·Clinical & Experimental Metastasis·I GitelmanJ C Roder
Jan 1, 1996·European Journal of Applied Physiology and Occupational Physiology·I K BrennerR J Shephard
Feb 1, 1991·Mycopathologia·R A Lindemann, C K Franker
Oct 1, 1982·Journal of Clinical Immunology·J KaplanJ Smith
Sep 1, 1988·Journal of Clinical Immunology·R MeehanR Goldblum
Mar 1, 1993·Journal of Clinical Immunology·M SchedlowskiR E Schmidt
Nov 1, 1993·Journal of Clinical Immunology·R McPhersonH F Pross
Sep 1, 1993·Journal of Clinical Immunology·M SchedlowskiR E Schmidt
Jan 1, 1996·Journal of Clinical Immunology·K OnoH Nawata
Jul 1, 1995·Journal of Clinical Immunology·K OnoH Nawata
Nov 1, 1996·Journal of Clinical Immunology·K OnoY Shimamoto
Jan 1, 1989·Cancer Immunology, Immunotherapy : CII·Y TokudaS H Golub
Jan 1, 1993·Cancer Immunology, Immunotherapy : CII·S M DubinettW H McBride
Jan 1, 1991·Cancer Immunology, Immunotherapy : CII·R A LindemannR K Gupta
Jan 1, 1990·Cancer Immunology, Immunotherapy : CII·S P HoW B Ershler
Jan 1, 1991·Cancer Immunology, Immunotherapy : CII·S G SwisherE C Holmes
Jan 1, 1993·Cancer Immunology, Immunotherapy : CII·E D Crum

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.