Spontaneous in vitro hatching of the human blastocyst: the proteomics of initially hatching cells.

In Vitro Cellular & Developmental Biology. Animal
Miriam AlmagorZeev Shoham

Abstract

Spontaneous in vitro hatching of human blastocysts starts with the formation of a tunnel through the zona pellucida (ZP) by cellular projections of trophoblast cells. Our aim was to identify the proteins that are upregulated in these initially hatching cells as compared to trophectoderm (TE) cells from blastocysts that had not yet hatched. Forty seven women that underwent assisted reproduction treatment donated their ICSI-derived polyploid blastocysts for the study. In polyploid blastocysts that started spontaneous hatching, hatched clusters of cells were collected from the outer side of the ZP. Liquid chromatography mass spectrometry was applied to determine the proteins that were upregulated in these cells as compared to TE cells obtained from inside the ZP. Whole non-hatched polyploid blastocysts were used as controls. Overall 1245 proteins were identified in all samples. Forty nine proteins were significantly upregulated in hatching cells and 17 in the TE cells. There was minimal overlap between hatching and TE samples; only serine protease inhibitors (SERPINS) and lipocalin were detected in both samples. Myosin and actin were highly upregulated in the hatching cells as well as paraoxonase, N-acetylmuramoyl alanine amidase,...Continue Reading

References

Oct 22, 2003·Reproductive Biomedicine Online·Henry SathananthanSulochana Gunasheela
Mar 27, 2010·Chemico-biological Interactions·C E FurlongC P Zabetian
Jul 20, 2010·Reproductive Sciences·Sarah NordqvistHelena Akerud
Aug 6, 2010·Biology of Reproduction·Kylie R DunningRebecca L Robker
Feb 18, 2011·Fertility and Sterility·Susanna McReynoldsMandy G Katz-Jaffe
Jul 6, 2012·Human Reproduction·Thorir HardarsonGayle Jones
Sep 25, 2012·Molecular Human Reproduction·Irene Tirado-GonzálezSandra M Blois
Jan 15, 2013·Human Reproduction·Gary F Clark
Aug 6, 2013·Fertility and Sterility·Kirstine KirkegaardHans Jakob Ingerslev
Nov 1, 2013·Human Genomics·Claire HeitVasilis Vasiliou
Oct 18, 2014·Current Molecular Medicine·J WangZ Shariat-Madar
Dec 27, 2015·American Journal of Reproductive Immunology : AJRI·Polani B SeshagiriPathak Madhulika
Aug 2, 2018·The Journal of Obstetrics and Gynaecology Research·Min Kyoung KimWoo Sik Lee
Sep 9, 2018·Methods in Molecular Biology·Alexandra LucasSriram Ambadapadi

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
acetylation
RNAseq

Software Mentioned

Andromeda

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.