PMID: 8589039Nov 1, 1995Paper

Spontaneous length variation in microsatellite DNA from human T-cell clones

Genes, Chromosomes & Cancer
P HackmanB Lambert

Abstract

Recently, much interest has been focused on instability of microsatellite DNA sequences such as di- and tri-nucleotide repeats in human cancers. Certain tumors show an increased frequency of mutation leading to repeat length variation at microsatellite loci, and it is thought that such instability may be a marker for the transformed phenotype. However, the spontaneous frequency by which repetitive DNA such as CA-repeats undergoes size changes in normal human somatic cells is not known. Therefore, it is not possible to decide if there is an increase in the frequency of microsatellite mutation in specific tumors or if the change observed simply reflects the frequency of microsatellite mutation in the cell population from which the tumor originates. To investigate this we have established panels of T-lymphocyte clones from 28 healthy males and determined the spontaneous length variations at three CA-repeat markers that are often used to investigate satellite instability: D2S123, D9S180, and D10S197. We found 3 T-cell clones with altered microsatellite size in a total of 178. This corresponds to a background frequency of 3 somatic microsatellite mutations in 1,028 alleles studied, i.e., 2.9 x 10(-3). This frequency is comparable to...Continue Reading

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Citations

Sep 28, 1998·Mutation Research·B LambertA Wennborg
Dec 16, 1998·Mutation Research·J CaoJ R Stringer
Sep 1, 1996·The Journal of Clinical Investigation·C L RosenbergP S Larson
Feb 13, 2013·ISRN Oncology·Michael J MonumentRl Tx Randall
Aug 15, 2012·Cytogenetic and Genome Research·P L PerelmanA S Graphodatsky
Jun 26, 1998·Annals of Surgical Oncology·S S MartinH S Schwartz

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