Spontaneous lethal sectoring, a further feature of Escherichia coli strains deficient in the function of rec and uvr genes.

Journal of Bacteriology
K Haefner

Abstract

Eight recombination-deficient (Rec(-)) mutants of Escherichia coli were studied. Progeny lines were obtained on solid media, by means of micromanipulation, and the colony-forming ability of individual cells was analyzed. Cells of all eight strains gave rise to colony-forming as well as non-colony-forming descendants ("lethal sectoring"). Lethal sectors, i.e., groups of non-colony-forming cells which originate from a common ancestor, appeared with frequencies per generation ranging between 4 and 20% in Rec(-) strains, whereas lethal sectors were rare in Rec(+) strains (less than 1%). A strain carrying a mutation (uvrA6) in one of the genes involved in pyrimidine dimer excision from deoxyribonucleic acid (DNA) showed twice as many lethal sectors per generation as a strain with the genotype uvrA(+). Similarly, a double mutant (AB2480, uvrA6, recA13) showed twice as much spontaneous lethal sectoring as the corresponding Rec(-) strain (uvrA(+), recA13). The kinetics of growth curves obtained in nutrient broth and the frequency of non-colony-forming units in stationary-phase broth cultures indicate clearly that lethal sectors occur in liquid cultures too. The causes for spontaneous lethal sectoring are unknown at present. It seems re...Continue Reading

References

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Citations

Sep 25, 2007·Chemistry & Biodiversity·Robin Holliday
Jan 1, 1970·Molecular & General Genetics : MGG·A L Schroeder
Jan 1, 1972·Humangenetik·G Mohn, F E Würgler
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Jul 1, 2020·Antimicrobial Agents and Chemotherapy·Nikola OjkicBartlomiej Waclaw
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